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MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE

The initial interaction between migration inhibitory factor (MIF) and the guinea pig alveolar and peritoneal macrophage was studied. MIF-containing supernatants were generated from sensitized lymph node lymphocytes obtained from guinea pigs immunized with bovine gamma globulin in complete Freund...

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Autores principales: Leu, Richard W., Eddleston, A. L. W. F., Hadden, John W., Good, Robert A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139265/
https://www.ncbi.nlm.nih.gov/pubmed/4115133
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author Leu, Richard W.
Eddleston, A. L. W. F.
Hadden, John W.
Good, Robert A.
author_facet Leu, Richard W.
Eddleston, A. L. W. F.
Hadden, John W.
Good, Robert A.
author_sort Leu, Richard W.
collection PubMed
description The initial interaction between migration inhibitory factor (MIF) and the guinea pig alveolar and peritoneal macrophage was studied. MIF-containing supernatants were generated from sensitized lymph node lymphocytes obtained from guinea pigs immunized with bovine gamma globulin in complete Freund's adjuvant. MIF-containing supernatants were markedly inhibitory for the migration of the peritoneal macrophage but had no effect on the alveolar macrophage. A linear relationship was observed between per cent inhibition of migration and serial twofold dilution of supernatant. Reexpressed in arbitrary MIF units, this relationship reflects a dose-response relationship with saturation characteristics. Pulse exposure of peritoneal macrophages to MIF resulted in adsorption of MIF onto both viable and nonviable cells with corresponding depletion of supernatant MIF. The alveolar macrophage did not adsorb MIF. Pulse adsorption of MIF onto the peritoneal macrophage is dependent on time, temperature, and cell number. Pretreatment of the cells with proteolytic enzyme prevents the adsorption of MIF while leaving migration unaffected. These observations support the existence of a specific cell surface receptor for MIF. The existence of such a receptor provides selectivity of immune modulation of macrophage populations by lymphocytes in delayed hypersensitivity reactions.
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spelling pubmed-21392652008-04-17 MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE Leu, Richard W. Eddleston, A. L. W. F. Hadden, John W. Good, Robert A. J Exp Med Article The initial interaction between migration inhibitory factor (MIF) and the guinea pig alveolar and peritoneal macrophage was studied. MIF-containing supernatants were generated from sensitized lymph node lymphocytes obtained from guinea pigs immunized with bovine gamma globulin in complete Freund's adjuvant. MIF-containing supernatants were markedly inhibitory for the migration of the peritoneal macrophage but had no effect on the alveolar macrophage. A linear relationship was observed between per cent inhibition of migration and serial twofold dilution of supernatant. Reexpressed in arbitrary MIF units, this relationship reflects a dose-response relationship with saturation characteristics. Pulse exposure of peritoneal macrophages to MIF resulted in adsorption of MIF onto both viable and nonviable cells with corresponding depletion of supernatant MIF. The alveolar macrophage did not adsorb MIF. Pulse adsorption of MIF onto the peritoneal macrophage is dependent on time, temperature, and cell number. Pretreatment of the cells with proteolytic enzyme prevents the adsorption of MIF while leaving migration unaffected. These observations support the existence of a specific cell surface receptor for MIF. The existence of such a receptor provides selectivity of immune modulation of macrophage populations by lymphocytes in delayed hypersensitivity reactions. The Rockefeller University Press 1972-09-01 /pmc/articles/PMC2139265/ /pubmed/4115133 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Leu, Richard W.
Eddleston, A. L. W. F.
Hadden, John W.
Good, Robert A.
MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title_full MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title_fullStr MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title_full_unstemmed MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title_short MECHANISM OF ACTION OF MIGRATION INHIBITORY FACTOR (MIF) : I. EVIDENCE FOR A RECEPTOR FOR MIF PRESENT ON THE PERITONEAL MACROPHAGE BUT NOT ON THE ALVEOLAR MACROPHAGE
title_sort mechanism of action of migration inhibitory factor (mif) : i. evidence for a receptor for mif present on the peritoneal macrophage but not on the alveolar macrophage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139265/
https://www.ncbi.nlm.nih.gov/pubmed/4115133
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