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ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY
To study the occurrence of immunocompetent cells directly responsible for antibody production, cells from yolk sac, embryonic liver, bursa of Fabricius, bone marrow, spleen, or thymus were injected together with SRBC and Brucella abortus into 4-day old cyclophosphamide-treated chicks. A second stimu...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1972
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139285/ https://www.ncbi.nlm.nih.gov/pubmed/4403475 |
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author | Toivanen, Paavo Toivanen, Auli Good, Robert A. |
author_facet | Toivanen, Paavo Toivanen, Auli Good, Robert A. |
author_sort | Toivanen, Paavo |
collection | PubMed |
description | To study the occurrence of immunocompetent cells directly responsible for antibody production, cells from yolk sac, embryonic liver, bursa of Fabricius, bone marrow, spleen, or thymus were injected together with SRBC and Brucella abortus into 4-day old cyclophosphamide-treated chicks. A second stimulation was given 4 days later, and samples taken 4 days thereafter were used for antibody titrations. During ontogeny, immunocompetent cells appeared in significant numbers first in the spleen for anti-SRBC responses, but in the bursa for anti-Brucella responses. Later these cells were also found in thymus and bone marrow. In the bursa, cells immunocompetent for anti-SRBC response were not encountered in significant numbers. The slight response to SRBC by transferred bursa cells reflects the presence of stem cells and their immediate descendents in the bursa at different stages of development. These findings are compared with the development and maturation of the stem cell responsible for humoral immunity. In the bursa, development of the stem cell population precedes that of immunocompetent cells. The opposite relationship was found in bone marrow, spleen, and thymus where immunocompetent cells were always present some weeks before the appearance of cells capable of achieving a long-lasting reconstitution of bursa-dependent functions. These observations reveal that the bursa seeds out immunocompetent cells during its entire postembryonic development, but does not release the lymphoid stem cell population before this population has matured sufficiently and before the bursa itself, after fulfilling its function, starts to involute. |
format | Text |
id | pubmed-2139285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1972 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21392852008-04-17 ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY Toivanen, Paavo Toivanen, Auli Good, Robert A. J Exp Med Article To study the occurrence of immunocompetent cells directly responsible for antibody production, cells from yolk sac, embryonic liver, bursa of Fabricius, bone marrow, spleen, or thymus were injected together with SRBC and Brucella abortus into 4-day old cyclophosphamide-treated chicks. A second stimulation was given 4 days later, and samples taken 4 days thereafter were used for antibody titrations. During ontogeny, immunocompetent cells appeared in significant numbers first in the spleen for anti-SRBC responses, but in the bursa for anti-Brucella responses. Later these cells were also found in thymus and bone marrow. In the bursa, cells immunocompetent for anti-SRBC response were not encountered in significant numbers. The slight response to SRBC by transferred bursa cells reflects the presence of stem cells and their immediate descendents in the bursa at different stages of development. These findings are compared with the development and maturation of the stem cell responsible for humoral immunity. In the bursa, development of the stem cell population precedes that of immunocompetent cells. The opposite relationship was found in bone marrow, spleen, and thymus where immunocompetent cells were always present some weeks before the appearance of cells capable of achieving a long-lasting reconstitution of bursa-dependent functions. These observations reveal that the bursa seeds out immunocompetent cells during its entire postembryonic development, but does not release the lymphoid stem cell population before this population has matured sufficiently and before the bursa itself, after fulfilling its function, starts to involute. The Rockefeller University Press 1972-10-01 /pmc/articles/PMC2139285/ /pubmed/4403475 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Toivanen, Paavo Toivanen, Auli Good, Robert A. ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title | ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title_full | ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title_fullStr | ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title_full_unstemmed | ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title_short | ONTOGENY OF BURSAL FUNCTION IN CHICKEN : III. IMMUNOCOMPETENT CELL FOR HUMORAL IMMUNITY |
title_sort | ontogeny of bursal function in chicken : iii. immunocompetent cell for humoral immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139285/ https://www.ncbi.nlm.nih.gov/pubmed/4403475 |
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