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NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE

Thymus cells from CBA and BALB/c mice are stimulated by syngeneic peripheral lymphoid cells in a "one-way" mixed lymphocyte reaction. The stimulating cell appears to be a mature B cell. Spleen cells from neonatal mice and thymus cells or bone marrow cells from adult mice are not able to in...

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Detalles Bibliográficos
Autores principales: von Boehmer, H., Shortman, K., Adams, Ann P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139322/
https://www.ncbi.nlm.nih.gov/pubmed/4539312
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author von Boehmer, H.
Shortman, K.
Adams, Ann P.
author_facet von Boehmer, H.
Shortman, K.
Adams, Ann P.
author_sort von Boehmer, H.
collection PubMed
description Thymus cells from CBA and BALB/c mice are stimulated by syngeneic peripheral lymphoid cells in a "one-way" mixed lymphocyte reaction. The stimulating cell appears to be a mature B cell. Spleen cells from neonatal mice and thymus cells or bone marrow cells from adult mice are not able to induce DNA synthesis in syngeneic thymus cells, although they stimulate significantly allogeneic thymocytes. The ability of peripheral B cells to serve as stimulating cell in a syngeneic reaction develops with the age of the animal. The marginal stimulation of syngeneic thymus cells when 90% pure peripheral T cells were used as stimulating cells indicated that T cells alone were ineffective in stimulating in syngeneic mixed lymphocyte reaction. However they stimulated effective allogeneic thymocytes. On a cell-to-cell basis, light density splenic lymphocytes stimulated both syngeneic and allogeneic thymocytes better than did more dense lymphocytes. The data obtained suggest that stimuli other than those responsible for allogeneic stimulation induce proliferation of syngeneic thymus cells under identical culture conditions.
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spelling pubmed-21393222008-04-17 NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE von Boehmer, H. Shortman, K. Adams, Ann P. J Exp Med Article Thymus cells from CBA and BALB/c mice are stimulated by syngeneic peripheral lymphoid cells in a "one-way" mixed lymphocyte reaction. The stimulating cell appears to be a mature B cell. Spleen cells from neonatal mice and thymus cells or bone marrow cells from adult mice are not able to induce DNA synthesis in syngeneic thymus cells, although they stimulate significantly allogeneic thymocytes. The ability of peripheral B cells to serve as stimulating cell in a syngeneic reaction develops with the age of the animal. The marginal stimulation of syngeneic thymus cells when 90% pure peripheral T cells were used as stimulating cells indicated that T cells alone were ineffective in stimulating in syngeneic mixed lymphocyte reaction. However they stimulated effective allogeneic thymocytes. On a cell-to-cell basis, light density splenic lymphocytes stimulated both syngeneic and allogeneic thymocytes better than did more dense lymphocytes. The data obtained suggest that stimuli other than those responsible for allogeneic stimulation induce proliferation of syngeneic thymus cells under identical culture conditions. The Rockefeller University Press 1972-11-30 /pmc/articles/PMC2139322/ /pubmed/4539312 Text en Copyright © 1972 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
von Boehmer, H.
Shortman, K.
Adams, Ann P.
NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title_full NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title_fullStr NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title_full_unstemmed NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title_short NATURE OF THE STIMULATING CELL IN THE SYNGENEIC AND THE ALLOGENEIC MIXED LYMPHOCYTE REACTION IN MICE
title_sort nature of the stimulating cell in the syngeneic and the allogeneic mixed lymphocyte reaction in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139322/
https://www.ncbi.nlm.nih.gov/pubmed/4539312
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