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ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW

Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F(1) mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected. Spleen, lymph...

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Autores principales: Herzenberg, Leonore A., Chan, Eva L., Ravitch, Myrnice M., Riblet, Roy J., Herzenberg, Leonard A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139347/
https://www.ncbi.nlm.nih.gov/pubmed/4541122
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author Herzenberg, Leonore A.
Chan, Eva L.
Ravitch, Myrnice M.
Riblet, Roy J.
Herzenberg, Leonard A.
author_facet Herzenberg, Leonore A.
Chan, Eva L.
Ravitch, Myrnice M.
Riblet, Roy J.
Herzenberg, Leonard A.
author_sort Herzenberg, Leonore A.
collection PubMed
description Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F(1) mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected. Spleen, lymph node, bone marrow, or thymus cells from suppressed mice suppress production of Ig-1b by syngeneic spleen cells from normal F(1) mice. When a mixture of suppressed and normal cells is transferred into lethally irradiated BALB/c mice, there is a short burst of Ig-1b production after which Ig-1b levels in the recipient fall rapidly below detectability. Pretreatment of the cells from the suppressed mice with antiserum specific for T cells (anti-Thy-1b) plus complement before mixture destroys the suppressing activity. Similar results with suppressor cells were obtained in vitro using Mishell-Dutton cultures. Mixture of spleen cells from suppressed animals with sheep erythrocyte (SRBC)-primed syngeneic normal spleen before culture suppresses Ig-1b plaque-forming cell (PFC) formation while leaving Ig-1a PFC unaffected. Treatment of the suppressed spleen with anti-Thy-1b before transfer removes the suppressing activity.
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spelling pubmed-21393472008-04-17 ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW Herzenberg, Leonore A. Chan, Eva L. Ravitch, Myrnice M. Riblet, Roy J. Herzenberg, Leonard A. J Exp Med Article Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F(1) mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected. Spleen, lymph node, bone marrow, or thymus cells from suppressed mice suppress production of Ig-1b by syngeneic spleen cells from normal F(1) mice. When a mixture of suppressed and normal cells is transferred into lethally irradiated BALB/c mice, there is a short burst of Ig-1b production after which Ig-1b levels in the recipient fall rapidly below detectability. Pretreatment of the cells from the suppressed mice with antiserum specific for T cells (anti-Thy-1b) plus complement before mixture destroys the suppressing activity. Similar results with suppressor cells were obtained in vitro using Mishell-Dutton cultures. Mixture of spleen cells from suppressed animals with sheep erythrocyte (SRBC)-primed syngeneic normal spleen before culture suppresses Ig-1b plaque-forming cell (PFC) formation while leaving Ig-1a PFC unaffected. Treatment of the suppressed spleen with anti-Thy-1b before transfer removes the suppressing activity. The Rockefeller University Press 1973-06-01 /pmc/articles/PMC2139347/ /pubmed/4541122 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Herzenberg, Leonore A.
Chan, Eva L.
Ravitch, Myrnice M.
Riblet, Roy J.
Herzenberg, Leonard A.
ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title_full ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title_fullStr ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title_full_unstemmed ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title_short ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW
title_sort active suppression of immunoglobulin allotype synthesis : iii. identitication of t cells as responsible for suppression by cells from spleen, thymus, lymph node, and bone marrow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139347/
https://www.ncbi.nlm.nih.gov/pubmed/4541122
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