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PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released duri...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1973
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139355/ https://www.ncbi.nlm.nih.gov/pubmed/4709268 |
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author | Weksler, Babette B. Coupal, C. Elaine |
author_facet | Weksler, Babette B. Coupal, C. Elaine |
author_sort | Weksler, Babette B. |
collection | PubMed |
description | A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released during blood clotting. Heated serum did not support the platelet-dependent generation of chemotactic activity. Treatment of fresh serum with antibody to the fifth component of complement also prevented development of activity. Purified human C5 but not C3 yielded chemotactic activity upon incubation with the platelet factor. Thus, human platelets are capable of stimulating chemotaxis via complement activation in a manner similar to leukocytes, and may therefore participate in the early stages of inflammation. |
format | Text |
id | pubmed-2139355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1973 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21393552008-04-17 PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM Weksler, Babette B. Coupal, C. Elaine J Exp Med Article A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released during blood clotting. Heated serum did not support the platelet-dependent generation of chemotactic activity. Treatment of fresh serum with antibody to the fifth component of complement also prevented development of activity. Purified human C5 but not C3 yielded chemotactic activity upon incubation with the platelet factor. Thus, human platelets are capable of stimulating chemotaxis via complement activation in a manner similar to leukocytes, and may therefore participate in the early stages of inflammation. The Rockefeller University Press 1973-06-01 /pmc/articles/PMC2139355/ /pubmed/4709268 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Weksler, Babette B. Coupal, C. Elaine PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title | PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title_full | PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title_fullStr | PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title_full_unstemmed | PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title_short | PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM |
title_sort | platelet-dependent generation of chemotactic activity in serum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139355/ https://www.ncbi.nlm.nih.gov/pubmed/4709268 |
work_keys_str_mv | AT wekslerbabetteb plateletdependentgenerationofchemotacticactivityinserum AT coupalcelaine plateletdependentgenerationofchemotacticactivityinserum |