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PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM

A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released duri...

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Detalles Bibliográficos
Autores principales: Weksler, Babette B., Coupal, C. Elaine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139355/
https://www.ncbi.nlm.nih.gov/pubmed/4709268
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author Weksler, Babette B.
Coupal, C. Elaine
author_facet Weksler, Babette B.
Coupal, C. Elaine
author_sort Weksler, Babette B.
collection PubMed
description A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released during blood clotting. Heated serum did not support the platelet-dependent generation of chemotactic activity. Treatment of fresh serum with antibody to the fifth component of complement also prevented development of activity. Purified human C5 but not C3 yielded chemotactic activity upon incubation with the platelet factor. Thus, human platelets are capable of stimulating chemotaxis via complement activation in a manner similar to leukocytes, and may therefore participate in the early stages of inflammation.
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spelling pubmed-21393552008-04-17 PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM Weksler, Babette B. Coupal, C. Elaine J Exp Med Article A protein fraction extracted from the lysosomal granules of human platelets generated chemotactic activity for polymorphonuclear leukocytes when incubated with fresh serum. The platelet factor was also released during platelet aggregation with collagen or epinephrine and appeared to be released during blood clotting. Heated serum did not support the platelet-dependent generation of chemotactic activity. Treatment of fresh serum with antibody to the fifth component of complement also prevented development of activity. Purified human C5 but not C3 yielded chemotactic activity upon incubation with the platelet factor. Thus, human platelets are capable of stimulating chemotaxis via complement activation in a manner similar to leukocytes, and may therefore participate in the early stages of inflammation. The Rockefeller University Press 1973-06-01 /pmc/articles/PMC2139355/ /pubmed/4709268 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Weksler, Babette B.
Coupal, C. Elaine
PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title_full PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title_fullStr PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title_full_unstemmed PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title_short PLATELET-DEPENDENT GENERATION OF CHEMOTACTIC ACTIVITY IN SERUM
title_sort platelet-dependent generation of chemotactic activity in serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139355/
https://www.ncbi.nlm.nih.gov/pubmed/4709268
work_keys_str_mv AT wekslerbabetteb plateletdependentgenerationofchemotacticactivityinserum
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