INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE

The interaction of histocompatibility (HL-A) antibodies and complement with synchronized human lymphoid cells in continuous culture has been investigated. The sensitivity of cultured lymphoid cells to HL-A antibody-mediated lysis in the cytotoxic test, the extent of activation of the complement syst...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrone, S., Cooper, N. R., Pellegrino, M. A., Reisfeld, R. A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139359/
https://www.ncbi.nlm.nih.gov/pubmed/4734592
_version_ 1782143777774764032
author Ferrone, S.
Cooper, N. R.
Pellegrino, M. A.
Reisfeld, R. A.
author_facet Ferrone, S.
Cooper, N. R.
Pellegrino, M. A.
Reisfeld, R. A.
author_sort Ferrone, S.
collection PubMed
description The interaction of histocompatibility (HL-A) antibodies and complement with synchronized human lymphoid cells in continuous culture has been investigated. The sensitivity of cultured lymphoid cells to HL-A antibody-mediated lysis in the cytotoxic test, the extent of activation of the complement system, the degree to which labeled complement components are bound, and the ability of these cells to absorb HL-A alloantibodies do not vary significantly during the cell growth cycle. The constancy of histocompatibility antigen expression throughout the growth cycle of cultured cells suggests that these cell surface markers are an essential part of membrane cytoarchitecture and could well play a critical role in determining the normal function of the cell membrane.
format Text
id pubmed-2139359
institution National Center for Biotechnology Information
language English
publishDate 1973
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21393592008-04-17 INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE Ferrone, S. Cooper, N. R. Pellegrino, M. A. Reisfeld, R. A. J Exp Med Article The interaction of histocompatibility (HL-A) antibodies and complement with synchronized human lymphoid cells in continuous culture has been investigated. The sensitivity of cultured lymphoid cells to HL-A antibody-mediated lysis in the cytotoxic test, the extent of activation of the complement system, the degree to which labeled complement components are bound, and the ability of these cells to absorb HL-A alloantibodies do not vary significantly during the cell growth cycle. The constancy of histocompatibility antigen expression throughout the growth cycle of cultured cells suggests that these cell surface markers are an essential part of membrane cytoarchitecture and could well play a critical role in determining the normal function of the cell membrane. The Rockefeller University Press 1973-01-01 /pmc/articles/PMC2139359/ /pubmed/4734592 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ferrone, S.
Cooper, N. R.
Pellegrino, M. A.
Reisfeld, R. A.
INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title_full INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title_fullStr INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title_full_unstemmed INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title_short INTERACTION OF HISTOCOMPATIBILITY (HL-A) ANTIBODIES AND COMPLEMENT WITH SYNCHRONIZED HUMAN LYMPHOID CELLS IN CONTINUOUS CULTURE
title_sort interaction of histocompatibility (hl-a) antibodies and complement with synchronized human lymphoid cells in continuous culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139359/
https://www.ncbi.nlm.nih.gov/pubmed/4734592
work_keys_str_mv AT ferrones interactionofhistocompatibilityhlaantibodiesandcomplementwithsynchronizedhumanlymphoidcellsincontinuousculture
AT coopernr interactionofhistocompatibilityhlaantibodiesandcomplementwithsynchronizedhumanlymphoidcellsincontinuousculture
AT pellegrinoma interactionofhistocompatibilityhlaantibodiesandcomplementwithsynchronizedhumanlymphoidcellsincontinuousculture
AT reisfeldra interactionofhistocompatibilityhlaantibodiesandcomplementwithsynchronizedhumanlymphoidcellsincontinuousculture

Ejemplares similares