REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN

The specificity of antigen recognition by thymus-derived helper cells (T cells) and antibody was examined in mice, heterologous erythrocyte antigens from sheep (SRBC), goat (GRBC), burro (BRBC), chicken (CRBC), and toad (TRBC) being used. Antibody specificity was tested by a number of functional ass...

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Detalles Bibliográficos
Autores principales: Hoffmann, Michael, Kappler, John W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139373/
https://www.ncbi.nlm.nih.gov/pubmed/4120287
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author Hoffmann, Michael
Kappler, John W.
author_facet Hoffmann, Michael
Kappler, John W.
author_sort Hoffmann, Michael
collection PubMed
description The specificity of antigen recognition by thymus-derived helper cells (T cells) and antibody was examined in mice, heterologous erythrocyte antigens from sheep (SRBC), goat (GRBC), burro (BRBC), chicken (CRBC), and toad (TRBC) being used. Antibody specificity was tested by a number of functional assays: hemagglutination, hemolysis, and immune suppression. The specificity of T cells was determined by titrating their ability to help the in vitro antitrinitrophenol (TNP) responses of mouse spleen cultures immunized with the hapten coupled to the various test erythrocytes as carrier. Anti-SRBC antibody cross-reacted with GRBC, but not with BRBC, CRBC, or TRBC. In contrast, SRBC-primed helper T cells cross-reacted with both GRBC and BRBC, but not with CRBC or TRBC, indicating a difference in the specificity of antigen recognition between the cellular and the humoral immune responses.
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spelling pubmed-21393732008-04-17 REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN Hoffmann, Michael Kappler, John W. J Exp Med Article The specificity of antigen recognition by thymus-derived helper cells (T cells) and antibody was examined in mice, heterologous erythrocyte antigens from sheep (SRBC), goat (GRBC), burro (BRBC), chicken (CRBC), and toad (TRBC) being used. Antibody specificity was tested by a number of functional assays: hemagglutination, hemolysis, and immune suppression. The specificity of T cells was determined by titrating their ability to help the in vitro antitrinitrophenol (TNP) responses of mouse spleen cultures immunized with the hapten coupled to the various test erythrocytes as carrier. Anti-SRBC antibody cross-reacted with GRBC, but not with BRBC, CRBC, or TRBC. In contrast, SRBC-primed helper T cells cross-reacted with both GRBC and BRBC, but not with CRBC or TRBC, indicating a difference in the specificity of antigen recognition between the cellular and the humoral immune responses. The Rockefeller University Press 1973-02-28 /pmc/articles/PMC2139373/ /pubmed/4120287 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hoffmann, Michael
Kappler, John W.
REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title_full REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title_fullStr REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title_full_unstemmed REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title_short REGULATION OF THE IMMUNE RESPONSE : II. QUALITATIVE AND QUANTITATIVE DIFFERENCES BETWEEN THYMUS- AND BONE MARROW-DERIVED LYMPHOCYTES IN THE RECOGNITION OF ANTIGEN
title_sort regulation of the immune response : ii. qualitative and quantitative differences between thymus- and bone marrow-derived lymphocytes in the recognition of antigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139373/
https://www.ncbi.nlm.nih.gov/pubmed/4120287
work_keys_str_mv AT hoffmannmichael regulationoftheimmuneresponseiiqualitativeandquantitativedifferencesbetweenthymusandbonemarrowderivedlymphocytesintherecognitionofantigen
AT kapplerjohnw regulationoftheimmuneresponseiiqualitativeandquantitativedifferencesbetweenthymusandbonemarrowderivedlymphocytesintherecognitionofantigen

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