A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES

Immunization of rabbits from a closed colony with streptococcal Group A-variant vaccines identified about two-thirds of them as low and heterogeneous responders. One-third of the rabbits showed a restriction of the response independent from the magnitude. Selective breeding from one monoclonal high-...

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Autores principales: Braun, Dietmar G., Kjems, Ebbe, Cramer, Matthias
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139410/
https://www.ncbi.nlm.nih.gov/pubmed/4727916
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author Braun, Dietmar G.
Kjems, Ebbe
Cramer, Matthias
author_facet Braun, Dietmar G.
Kjems, Ebbe
Cramer, Matthias
author_sort Braun, Dietmar G.
collection PubMed
description Immunization of rabbits from a closed colony with streptococcal Group A-variant vaccines identified about two-thirds of them as low and heterogeneous responders. One-third of the rabbits showed a restriction of the response independent from the magnitude. Selective breeding from one monoclonal high-responder male and two restricted high-responder female rabbits succeeded in segregation of high-responder progeny after two generations. Their antibody levels were on the average 2.5 times higher than those of the random group of rabbits and a small group of low-responder offspring. Immunization of 13 offspring originating from rabbits bred for restricted high response to the streptococcal Group C polysaccharide revealed that 11 progeny were restricted high responders and 2 progeny monoclonal high responders. This finding suggests that high responsiveness to the Groups A-variant and C polysaccharides is inherited as genetically linked traits. Selective breeding combinations between restricted and monoclonal high-responder rabbits by brother-sister matings succeeded in narrowing the isoelectric focusing spectra of Group A-variant-specific antibodies in the offspring. It furthermore revealed a preferential expression of monoclonal antibodies after three generations with a similar net charge as those identified first in the original monoclonal paternal parent. These data suggest that similar copies of structural genes for the variable regions are transmitted from the parent to the progeny.
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spelling pubmed-21394102008-04-17 A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES Braun, Dietmar G. Kjems, Ebbe Cramer, Matthias J Exp Med Article Immunization of rabbits from a closed colony with streptococcal Group A-variant vaccines identified about two-thirds of them as low and heterogeneous responders. One-third of the rabbits showed a restriction of the response independent from the magnitude. Selective breeding from one monoclonal high-responder male and two restricted high-responder female rabbits succeeded in segregation of high-responder progeny after two generations. Their antibody levels were on the average 2.5 times higher than those of the random group of rabbits and a small group of low-responder offspring. Immunization of 13 offspring originating from rabbits bred for restricted high response to the streptococcal Group C polysaccharide revealed that 11 progeny were restricted high responders and 2 progeny monoclonal high responders. This finding suggests that high responsiveness to the Groups A-variant and C polysaccharides is inherited as genetically linked traits. Selective breeding combinations between restricted and monoclonal high-responder rabbits by brother-sister matings succeeded in narrowing the isoelectric focusing spectra of Group A-variant-specific antibodies in the offspring. It furthermore revealed a preferential expression of monoclonal antibodies after three generations with a similar net charge as those identified first in the original monoclonal paternal parent. These data suggest that similar copies of structural genes for the variable regions are transmitted from the parent to the progeny. The Rockefeller University Press 1973-09-01 /pmc/articles/PMC2139410/ /pubmed/4727916 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Braun, Dietmar G.
Kjems, Ebbe
Cramer, Matthias
A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title_full A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title_fullStr A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title_full_unstemmed A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title_short A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES
title_sort rabbit family of restricted high responders to the streptococcal group a-variant polysaccharide : selective breeding narrows the isoelectric focusing spectra of dominant clones
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139410/
https://www.ncbi.nlm.nih.gov/pubmed/4727916
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