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FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION
Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth o...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1973
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139430/ https://www.ncbi.nlm.nih.gov/pubmed/4355423 |
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author | Ossowski, L. Quigley, J. P. Kellerman, G. M. Reich, E. |
author_facet | Ossowski, L. Quigley, J. P. Kellerman, G. M. Reich, E. |
author_sort | Ossowski, L. |
collection | PubMed |
description | Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth of normal and SV-40-transformed hamster embryo fibroblasts in liquid medium, (b) growth of SV-40-transformed hamster embryo fibroblasts in soft agar, (c) aggregation — a characteristic morphological change of SV-40-transformed hamster cells, and (d) migration of SV-40-transformed and control 3T3 cells from a monolayer into a "wound." The results demonstrated that exponential growth of both normal and transformed cells in liquid medium proceeded at the same rate in the presence or absence of plasminogen. In contrast, removal of plasminogen markedly depressed the plating efficiency of transformed cells in soft agar, eliminated their characteristic aggregation, and substantially reduced the extent of migration. The role of plasminogen and its activation in oncogenic transformation is discussed. |
format | Text |
id | pubmed-2139430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1973 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21394302008-04-17 FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION Ossowski, L. Quigley, J. P. Kellerman, G. M. Reich, E. J Exp Med Article Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth of normal and SV-40-transformed hamster embryo fibroblasts in liquid medium, (b) growth of SV-40-transformed hamster embryo fibroblasts in soft agar, (c) aggregation — a characteristic morphological change of SV-40-transformed hamster cells, and (d) migration of SV-40-transformed and control 3T3 cells from a monolayer into a "wound." The results demonstrated that exponential growth of both normal and transformed cells in liquid medium proceeded at the same rate in the presence or absence of plasminogen. In contrast, removal of plasminogen markedly depressed the plating efficiency of transformed cells in soft agar, eliminated their characteristic aggregation, and substantially reduced the extent of migration. The role of plasminogen and its activation in oncogenic transformation is discussed. The Rockefeller University Press 1973-10-31 /pmc/articles/PMC2139430/ /pubmed/4355423 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Ossowski, L. Quigley, J. P. Kellerman, G. M. Reich, E. FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title | FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title_full | FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title_fullStr | FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title_full_unstemmed | FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title_short | FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATION : REQUIREMENT OF PLASMINOGEN FOR CORRELATED CHANGES IN CELLULAR MORPHOLOGY, COLONY FORMATION IN AGAR, AND CELL MIGRATION |
title_sort | fibrinolysis associated with oncogenic transformation : requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar, and cell migration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139430/ https://www.ncbi.nlm.nih.gov/pubmed/4355423 |
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