MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS

The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in th...

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Detalles Bibliográficos
Autores principales: Vaheri, Antti, Ruoslahti, Erkki, Sarvas, Matti, Nurminen, Marjatta
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139454/
https://www.ncbi.nlm.nih.gov/pubmed/4762551
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author Vaheri, Antti
Ruoslahti, Erkki
Sarvas, Matti
Nurminen, Marjatta
author_facet Vaheri, Antti
Ruoslahti, Erkki
Sarvas, Matti
Nurminen, Marjatta
author_sort Vaheri, Antti
collection PubMed
description The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in thymidine incorporation and cell number. The concentration of LPS maximally stimulating fibroblasts was remarkably low, about 0.1–1 ng/ml. LPS preparation with very different sugar chains gave quite similar results indicating that the architecture of the hydrophilic carbohydrate part is not critical for the mitogenic effect.
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spelling pubmed-21394542008-04-17 MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS Vaheri, Antti Ruoslahti, Erkki Sarvas, Matti Nurminen, Marjatta J Exp Med Article The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in thymidine incorporation and cell number. The concentration of LPS maximally stimulating fibroblasts was remarkably low, about 0.1–1 ng/ml. LPS preparation with very different sugar chains gave quite similar results indicating that the architecture of the hydrophilic carbohydrate part is not critical for the mitogenic effect. The Rockefeller University Press 1973-11-30 /pmc/articles/PMC2139454/ /pubmed/4762551 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Vaheri, Antti
Ruoslahti, Erkki
Sarvas, Matti
Nurminen, Marjatta
MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title_full MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title_fullStr MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title_full_unstemmed MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title_short MITOGENIC EFFECT BY LIPOPOLYSACCHARIDE AND POKEWEED LECTIN ON DENSITY-INHIBITED CHICK EMBRYO FIBROBLASTS
title_sort mitogenic effect by lipopolysaccharide and pokeweed lectin on density-inhibited chick embryo fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139454/
https://www.ncbi.nlm.nih.gov/pubmed/4762551
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AT sarvasmatti mitogeniceffectbylipopolysaccharideandpokeweedlectinondensityinhibitedchickembryofibroblasts
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