FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM

The role of C3 proactivator (C3PA) and a factor isolated from cobra venom (CoF) in the formation of a principle able to cleave C3 was investigated. The results clearly demonstrate two modes of interaction of C3PA with CoF. In isolated form, C3PA and CoF were found to form a reversible protein-protei...

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Autor principal: Cooper, Neil R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139485/
https://www.ncbi.nlm.nih.gov/pubmed/4734404
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author Cooper, Neil R.
author_facet Cooper, Neil R.
author_sort Cooper, Neil R.
collection PubMed
description The role of C3 proactivator (C3PA) and a factor isolated from cobra venom (CoF) in the formation of a principle able to cleave C3 was investigated. The results clearly demonstrate two modes of interaction of C3PA with CoF. In isolated form, C3PA and CoF were found to form a reversible protein-protein complex in free solution. This complex had some C3 cleaving activity. In the presence of minute amounts of a partially purified normal serum substance, factor D, the C3PA-CoF complex was stabilized and its efficiency in cleaving C3 was greatly increased. Factor D is thus an activator of C3PA. A firm complex composed of C3PA and CoF, and possessing C3 cleaving activity, was also formed by addition of CoF to serum.
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spelling pubmed-21394852008-04-17 FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM Cooper, Neil R. J Exp Med Article The role of C3 proactivator (C3PA) and a factor isolated from cobra venom (CoF) in the formation of a principle able to cleave C3 was investigated. The results clearly demonstrate two modes of interaction of C3PA with CoF. In isolated form, C3PA and CoF were found to form a reversible protein-protein complex in free solution. This complex had some C3 cleaving activity. In the presence of minute amounts of a partially purified normal serum substance, factor D, the C3PA-CoF complex was stabilized and its efficiency in cleaving C3 was greatly increased. Factor D is thus an activator of C3PA. A firm complex composed of C3PA and CoF, and possessing C3 cleaving activity, was also formed by addition of CoF to serum. The Rockefeller University Press 1973-01-31 /pmc/articles/PMC2139485/ /pubmed/4734404 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cooper, Neil R.
FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title_full FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title_fullStr FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title_full_unstemmed FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title_short FORMATION AND FUNCTION OF A COMPLEX OF THE C3 PROACTIVATOR WITH A PROTEIN FROM COBRA VENOM
title_sort formation and function of a complex of the c3 proactivator with a protein from cobra venom
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139485/
https://www.ncbi.nlm.nih.gov/pubmed/4734404
work_keys_str_mv AT cooperneilr formationandfunctionofacomplexofthec3proactivatorwithaproteinfromcobravenom

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