CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES

A procedure of intrafamilial immunization is described for production of antisera recognizing DL-A haplotypes. In a colony consisting of 1 sire, 6 bitches, and 67 offspring all haplotypes could be accurately allocated. In the colony the observed reaction frequencies of the antisera are in agreement...

Descripción completa

Detalles Bibliográficos
Autores principales: van der Does, J. A., van Rood, J. J., Walker, W. S., Epstein, R. B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139492/
https://www.ncbi.nlm.nih.gov/pubmed/4685706
_version_ 1782143808966754304
author van der Does, J. A.
van Rood, J. J.
Walker, W. S.
Epstein, R. B.
author_facet van der Does, J. A.
van Rood, J. J.
Walker, W. S.
Epstein, R. B.
author_sort van der Does, J. A.
collection PubMed
description A procedure of intrafamilial immunization is described for production of antisera recognizing DL-A haplotypes. In a colony consisting of 1 sire, 6 bitches, and 67 offspring all haplotypes could be accurately allocated. In the colony the observed reaction frequencies of the antisera are in agreement with mendelian codominant inheritance. Mixed lymphocyte culture tests confirmed the accuracy of the serologic typing and the presence of homozygous individuals within the colony. Further evidence is presented supporting the presence of two or more subloci within the DL-A system. Colonies of canines such as the one described should provide a sensitive system for evaluating interaction between serologic DL-A typing, MLC reactivity, and immune response genetics in a nonrodent species which is not highly inbred.
format Text
id pubmed-2139492
institution National Center for Biotechnology Information
language English
publishDate 1973
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21394922008-04-17 CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES van der Does, J. A. van Rood, J. J. Walker, W. S. Epstein, R. B. J Exp Med Article A procedure of intrafamilial immunization is described for production of antisera recognizing DL-A haplotypes. In a colony consisting of 1 sire, 6 bitches, and 67 offspring all haplotypes could be accurately allocated. In the colony the observed reaction frequencies of the antisera are in agreement with mendelian codominant inheritance. Mixed lymphocyte culture tests confirmed the accuracy of the serologic typing and the presence of homozygous individuals within the colony. Further evidence is presented supporting the presence of two or more subloci within the DL-A system. Colonies of canines such as the one described should provide a sensitive system for evaluating interaction between serologic DL-A typing, MLC reactivity, and immune response genetics in a nonrodent species which is not highly inbred. The Rockefeller University Press 1973-01-31 /pmc/articles/PMC2139492/ /pubmed/4685706 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
van der Does, J. A.
van Rood, J. J.
Walker, W. S.
Epstein, R. B.
CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title_full CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title_fullStr CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title_full_unstemmed CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title_short CONSEQUENT INTRAFAMILIAL IMMUNIZATION FOR DL-A HAPLOTYPING IN CANINES
title_sort consequent intrafamilial immunization for dl-a haplotyping in canines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139492/
https://www.ncbi.nlm.nih.gov/pubmed/4685706
work_keys_str_mv AT vanderdoesja consequentintrafamilialimmunizationfordlahaplotypingincanines
AT vanroodjj consequentintrafamilialimmunizationfordlahaplotypingincanines
AT walkerws consequentintrafamilialimmunizationfordlahaplotypingincanines
AT epsteinrb consequentintrafamilialimmunizationfordlahaplotypingincanines

Ejemplares similares