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THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION

Properdin (P), a highly basic euglobulin, was purified from human serum to molecular homogeneity without the use of zymosan. Isolated P was found to efficiently initiate activation of the alternate pathway of complement activation (C3 activator or properdin system) and to be an essential component d...

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Detalles Bibliográficos
Autores principales: Götze, Otto, Müller-Eberhard, Hans J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139515/
https://www.ncbi.nlm.nih.gov/pubmed/4808709
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author Götze, Otto
Müller-Eberhard, Hans J.
author_facet Götze, Otto
Müller-Eberhard, Hans J.
author_sort Götze, Otto
collection PubMed
description Properdin (P), a highly basic euglobulin, was purified from human serum to molecular homogeneity without the use of zymosan. Isolated P was found to efficiently initiate activation of the alternate pathway of complement activation (C3 activator or properdin system) and to be an essential component during its early reaction stages. The activity of isolated P did not require the presence of an activating polysaccharide. It was therefore concluded that purified P had been obtained in an activated form (P). In an isolated reaction system containing purified C3, C3 proactivator (C3PA), and C3 proactivator convertase (C3PAse), P was able to mediate the activation of C3PAse which in turn activated C3PA to cleave C3. This activation of C3PAse was found to depend on the presence of native C3. These results allowed the formulation of a concept in which P is envisaged to act as a modulator of native C3 enabling it to activate C3PAse. Activation of C3 was efficiently mediated by P in the fluid phase. Efficient activation of C5, however, required the participation of an insoluble polysaccharide (zymosan). The possibility is raised therefore that P might also be an integral part of the multimolecular C5 convertase of the alternate pathway of complement activation.
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spelling pubmed-21395152008-04-17 THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION Götze, Otto Müller-Eberhard, Hans J. J Exp Med Article Properdin (P), a highly basic euglobulin, was purified from human serum to molecular homogeneity without the use of zymosan. Isolated P was found to efficiently initiate activation of the alternate pathway of complement activation (C3 activator or properdin system) and to be an essential component during its early reaction stages. The activity of isolated P did not require the presence of an activating polysaccharide. It was therefore concluded that purified P had been obtained in an activated form (P). In an isolated reaction system containing purified C3, C3 proactivator (C3PA), and C3 proactivator convertase (C3PAse), P was able to mediate the activation of C3PAse which in turn activated C3PA to cleave C3. This activation of C3PAse was found to depend on the presence of native C3. These results allowed the formulation of a concept in which P is envisaged to act as a modulator of native C3 enabling it to activate C3PAse. Activation of C3 was efficiently mediated by P in the fluid phase. Efficient activation of C5, however, required the participation of an insoluble polysaccharide (zymosan). The possibility is raised therefore that P might also be an integral part of the multimolecular C5 convertase of the alternate pathway of complement activation. The Rockefeller University Press 1974-01-01 /pmc/articles/PMC2139515/ /pubmed/4808709 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Götze, Otto
Müller-Eberhard, Hans J.
THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title_full THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title_fullStr THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title_full_unstemmed THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title_short THE ROLE OF PROPERDIN IN THE ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION
title_sort role of properdin in the alternate pathway of complement activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139515/
https://www.ncbi.nlm.nih.gov/pubmed/4808709
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