SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES

The mixing of two histoincompatible human lymphocyte cell lines generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernates were subjected to exclusion chromatogr...

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Detalles Bibliográficos
Autores principales: Rubin, Arnold S., MacDonald, A. Bruce, Coons, Albert H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Brief Definitive Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139523/
https://www.ncbi.nlm.nih.gov/pubmed/4855755
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author Rubin, Arnold S.
MacDonald, A. Bruce
Coons, Albert H.
author_facet Rubin, Arnold S.
MacDonald, A. Bruce
Coons, Albert H.
author_sort Rubin, Arnold S.
collection PubMed
description The mixing of two histoincompatible human lymphocyte cell lines generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernates were subjected to exclusion chromatography on Sephadex G-150 and BioGel P-150, the active principle eluted with molecules of approximately 35,000 mol wt. Column aliquots from similarly treated supernates from independent cultures of each lymphoid cell line were inactive. The human enhancing factor was concentrated and purified by ammonium sulfate fractionation, followed by Sephadex gel filtration and polyacrylamide gel electrophoresis.
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spelling pubmed-21395232008-04-17 SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES Rubin, Arnold S. MacDonald, A. Bruce Coons, Albert H. J Exp Med Brief Definitive Reports The mixing of two histoincompatible human lymphocyte cell lines generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernates were subjected to exclusion chromatography on Sephadex G-150 and BioGel P-150, the active principle eluted with molecules of approximately 35,000 mol wt. Column aliquots from similarly treated supernates from independent cultures of each lymphoid cell line were inactive. The human enhancing factor was concentrated and purified by ammonium sulfate fractionation, followed by Sephadex gel filtration and polyacrylamide gel electrophoresis. The Rockefeller University Press 1974-02-01 /pmc/articles/PMC2139523/ /pubmed/4855755 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Rubin, Arnold S.
MacDonald, A. Bruce
Coons, Albert H.
SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title_full SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title_fullStr SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title_full_unstemmed SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title_short SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES
title_sort specific heterologous enhancement of immune responses : vi. partial purification of a nonspecific enhancing factor from supernates of allogeneically stimulated human lymphocyte cell lines
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139523/
https://www.ncbi.nlm.nih.gov/pubmed/4855755
work_keys_str_mv AT rubinarnolds specificheterologousenhancementofimmuneresponsesvipartialpurificationofanonspecificenhancingfactorfromsupernatesofallogeneicallystimulatedhumanlymphocytecelllines
AT macdonaldabruce specificheterologousenhancementofimmuneresponsesvipartialpurificationofanonspecificenhancingfactorfromsupernatesofallogeneicallystimulatedhumanlymphocytecelllines
AT coonsalberth specificheterologousenhancementofimmuneresponsesvipartialpurificationofanonspecificenhancingfactorfromsupernatesofallogeneicallystimulatedhumanlymphocytecelllines

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