HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY

Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24–72 h after exposure of cells to antibody. The stimulation of...

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Detalles Bibliográficos
Autores principales: Shearer, William T., Philpott, Gordon W., Parker, Charles W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139524/
https://www.ncbi.nlm.nih.gov/pubmed/4855754
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author Shearer, William T.
Philpott, Gordon W.
Parker, Charles W.
author_facet Shearer, William T.
Philpott, Gordon W.
Parker, Charles W.
author_sort Shearer, William T.
collection PubMed
description Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24–72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, ε-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth.
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spelling pubmed-21395242008-04-17 HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY Shearer, William T. Philpott, Gordon W. Parker, Charles W. J Exp Med Article Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24–72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, ε-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth. The Rockefeller University Press 1974-02-01 /pmc/articles/PMC2139524/ /pubmed/4855754 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shearer, William T.
Philpott, Gordon W.
Parker, Charles W.
HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title_full HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title_fullStr HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title_full_unstemmed HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title_short HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [(125)I]IODODEOXYURIDINE AND [(3)H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
title_sort humoral immunostimulation : i. increased uptake of [(125)i]iododeoxyuridine and [(3)h]thymidine into tnp-cells treated with anti-tnp antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139524/
https://www.ncbi.nlm.nih.gov/pubmed/4855754
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