AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO

A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precur...

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Detalles Bibliográficos
Autores principales: Hünig, T., Schimpl, A., Wecker, E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139546/
https://www.ncbi.nlm.nih.gov/pubmed/4544248
Descripción
Sumario:A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.

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