AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO

A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precur...

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Detalles Bibliográficos
Autores principales: Hünig, T., Schimpl, A., Wecker, E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139546/
https://www.ncbi.nlm.nih.gov/pubmed/4544248
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author Hünig, T.
Schimpl, A.
Wecker, E.
author_facet Hünig, T.
Schimpl, A.
Wecker, E.
author_sort Hünig, T.
collection PubMed
description A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.
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spelling pubmed-21395462008-04-17 AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO Hünig, T. Schimpl, A. Wecker, E. J Exp Med Article A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide. The Rockefeller University Press 1974-03-01 /pmc/articles/PMC2139546/ /pubmed/4544248 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hünig, T.
Schimpl, A.
Wecker, E.
AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title_full AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title_fullStr AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title_full_unstemmed AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title_short AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
title_sort autoradiographic studies on the proliferation of antibody-producing cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139546/
https://www.ncbi.nlm.nih.gov/pubmed/4544248
work_keys_str_mv AT hunigt autoradiographicstudiesontheproliferationofantibodyproducingcellsinvitro
AT schimpla autoradiographicstudiesontheproliferationofantibodyproducingcellsinvitro
AT weckere autoradiographicstudiesontheproliferationofantibodyproducingcellsinvitro

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