THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE

Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (µ and K) have molecular sizes of 75,000 and 33,000 daltons, respecti...

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Detalles Bibliográficos
Autor principal: Kennel, Stephen J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Brief Definitive Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139566/
https://www.ncbi.nlm.nih.gov/pubmed/4816301
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author Kennel, Stephen J.
author_facet Kennel, Stephen J.
author_sort Kennel, Stephen J.
collection PubMed
description Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (µ and K) have molecular sizes of 75,000 and 33,000 daltons, respectively. Further studies showed that [(3)H]galactose can be incorporated into K-chains, but that extra protein and not carbohydrate was responsible for the increased molecular size of this molecule.
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spelling pubmed-21395662008-04-17 THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE Kennel, Stephen J. J Exp Med Brief Definitive Reports Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (µ and K) have molecular sizes of 75,000 and 33,000 daltons, respectively. Further studies showed that [(3)H]galactose can be incorporated into K-chains, but that extra protein and not carbohydrate was responsible for the increased molecular size of this molecule. The Rockefeller University Press 1974-04-01 /pmc/articles/PMC2139566/ /pubmed/4816301 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Kennel, Stephen J.
THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title_full THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title_fullStr THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title_full_unstemmed THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title_short THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
title_sort k-chains of the immunoglobulin from a continuous culture of human lymphocytes (daudi) have an unusual molecular size
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139566/
https://www.ncbi.nlm.nih.gov/pubmed/4816301
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