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LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE

Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain anti...

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Detalles Bibliográficos
Autores principales: Jobin, M. E., Fahey, J. L., Price, Z.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139596/
https://www.ncbi.nlm.nih.gov/pubmed/4858717
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author Jobin, M. E.
Fahey, J. L.
Price, Z.
author_facet Jobin, M. E.
Fahey, J. L.
Price, Z.
author_sort Jobin, M. E.
collection PubMed
description Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons.
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spelling pubmed-21395962008-04-17 LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE Jobin, M. E. Fahey, J. L. Price, Z. J Exp Med Article Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons. The Rockefeller University Press 1974-08-01 /pmc/articles/PMC2139596/ /pubmed/4858717 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Jobin, M. E.
Fahey, J. L.
Price, Z.
LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title_full LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title_fullStr LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title_full_unstemmed LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title_short LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA : I. REQUIREMENT OF A PLASMACYTE-STIMULATING FACTOR FOR THE PROLIFERATION OF MYELOMA CELLS IN TISSUE CULTURE
title_sort long-term establishment of a human plasmacyte cell line derived from a patient with igd multiple myeloma : i. requirement of a plasmacyte-stimulating factor for the proliferation of myeloma cells in tissue culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139596/
https://www.ncbi.nlm.nih.gov/pubmed/4858717
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