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SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS
In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates r...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1974
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139608/ https://www.ncbi.nlm.nih.gov/pubmed/4547307 |
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author | Ramseier, Hansruedy |
author_facet | Ramseier, Hansruedy |
author_sort | Ramseier, Hansruedy |
collection | PubMed |
description | In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates reproduced faithfully those found with the corresponding cells. Large amounts of receptors were released by untreated spleen cells and by spleen cells treated with a rabbit antimouse immunoglobulin serum and complement, smaller amounts were released by bone marrow cells, and native thymus cells released none. Spleen cells from nude mice and spleen cells from normal mice treated with anti-θ serum and complement showed no release of receptors. It was concluded that receptors active in the PAR test were of T-cell origin. Release of T-cell receptors was found to be discontinuous and proceeded in waves. The amount of released receptors depended on the number of cells cultivated. Release occurred at 37°C but not at 4°C. Interaction with antigen, however, was temperature-independent. In contrast to T-cell receptors, a release of H-2 antigens could not be detected with the culture conditions employed. |
format | Text |
id | pubmed-2139608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1974 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21396082008-04-17 SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS Ramseier, Hansruedy J Exp Med Article In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates reproduced faithfully those found with the corresponding cells. Large amounts of receptors were released by untreated spleen cells and by spleen cells treated with a rabbit antimouse immunoglobulin serum and complement, smaller amounts were released by bone marrow cells, and native thymus cells released none. Spleen cells from nude mice and spleen cells from normal mice treated with anti-θ serum and complement showed no release of receptors. It was concluded that receptors active in the PAR test were of T-cell origin. Release of T-cell receptors was found to be discontinuous and proceeded in waves. The amount of released receptors depended on the number of cells cultivated. Release occurred at 37°C but not at 4°C. Interaction with antigen, however, was temperature-independent. In contrast to T-cell receptors, a release of H-2 antigens could not be detected with the culture conditions employed. The Rockefeller University Press 1974-09-01 /pmc/articles/PMC2139608/ /pubmed/4547307 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Ramseier, Hansruedy SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title | SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title_full | SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title_fullStr | SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title_full_unstemmed | SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title_short | SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS : I. RECOGNITION OF ALLOANTIGENS AND RECEPTOR RELEASE DYNAMICS |
title_sort | spontaneous release of t-cell receptors for alloantigens : i. recognition of alloantigens and receptor release dynamics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139608/ https://www.ncbi.nlm.nih.gov/pubmed/4547307 |
work_keys_str_mv | AT ramseierhansruedy spontaneousreleaseoftcellreceptorsforalloantigensirecognitionofalloantigensandreceptorreleasedynamics |