INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants

The rates of immunoglobulin synthesis have been examined in two MFC-11 cell lines which were independently adapted to tissue culture and in light-chain-producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobu...

Descripción completa

Detalles Bibliográficos
Autores principales: Laskov, Reuven, Scharff, Matthew D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Brief Definitive Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139627/
https://www.ncbi.nlm.nih.gov/pubmed/4139223
_version_ 1782143840521551872
author Laskov, Reuven
Scharff, Matthew D.
author_facet Laskov, Reuven
Scharff, Matthew D.
author_sort Laskov, Reuven
collection PubMed
description The rates of immunoglobulin synthesis have been examined in two MFC-11 cell lines which were independently adapted to tissue culture and in light-chain-producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.
format Text
id pubmed-2139627
institution National Center for Biotechnology Information
language English
publishDate 1974
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21396272008-04-17 INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants Laskov, Reuven Scharff, Matthew D. J Exp Med Brief Definitive Reports The rates of immunoglobulin synthesis have been examined in two MFC-11 cell lines which were independently adapted to tissue culture and in light-chain-producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line. The Rockefeller University Press 1974-10-01 /pmc/articles/PMC2139627/ /pubmed/4139223 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Laskov, Reuven
Scharff, Matthew D.
INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title_full INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title_fullStr INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title_full_unstemmed INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title_short INDEPENDENT SYNTHESIS OF LIGHT AND HEAVY CHAINS : Quantitation of Light-Chain Production by Mouse Myeloma Variants
title_sort independent synthesis of light and heavy chains : quantitation of light-chain production by mouse myeloma variants
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139627/
https://www.ncbi.nlm.nih.gov/pubmed/4139223
work_keys_str_mv AT laskovreuven independentsynthesisoflightandheavychainsquantitationoflightchainproductionbymousemyelomavariants
AT scharffmatthewd independentsynthesisoflightandheavychainsquantitationoflightchainproductionbymousemyelomavariants

Ejemplares similares