UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES

Incubation of human leukocytes with dehydroascorbate (DHA) results in an increase in their reduced ascorbate (AA) content and hexose monophosphate shunt (HMS) activity, independent of oxygen supply. Incubation with AA induces these changes only in the presence of oxygen. The increase in HMS activity...

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Detalles Bibliográficos
Autores principales: Bigley, Robert H., Stankova, Libuse
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139671/
https://www.ncbi.nlm.nih.gov/pubmed/4825242
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author Bigley, Robert H.
Stankova, Libuse
author_facet Bigley, Robert H.
Stankova, Libuse
author_sort Bigley, Robert H.
collection PubMed
description Incubation of human leukocytes with dehydroascorbate (DHA) results in an increase in their reduced ascorbate (AA) content and hexose monophosphate shunt (HMS) activity, independent of oxygen supply. Incubation with AA induces these changes only in the presence of oxygen. The increase in HMS activity observed as cell AA increases by 1 µmol is the same during incubation with either DHA or AA. We propose that human leukocytes take up ascorbate as DHA (AA after oxidation to DHA) and reduce it promptly to AA, and that HMS stimulation upon incubation with either AA or DHA is a result of DHA reduction.
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spelling pubmed-21396712008-04-17 UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES Bigley, Robert H. Stankova, Libuse J Exp Med Article Incubation of human leukocytes with dehydroascorbate (DHA) results in an increase in their reduced ascorbate (AA) content and hexose monophosphate shunt (HMS) activity, independent of oxygen supply. Incubation with AA induces these changes only in the presence of oxygen. The increase in HMS activity observed as cell AA increases by 1 µmol is the same during incubation with either DHA or AA. We propose that human leukocytes take up ascorbate as DHA (AA after oxidation to DHA) and reduce it promptly to AA, and that HMS stimulation upon incubation with either AA or DHA is a result of DHA reduction. The Rockefeller University Press 1974-05-01 /pmc/articles/PMC2139671/ /pubmed/4825242 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bigley, Robert H.
Stankova, Libuse
UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title_full UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title_fullStr UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title_full_unstemmed UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title_short UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
title_sort uptake and reduction of oxidized and reduced ascorbate by human leukocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139671/
https://www.ncbi.nlm.nih.gov/pubmed/4825242
work_keys_str_mv AT bigleyroberth uptakeandreductionofoxidizedandreducedascorbatebyhumanleukocytes
AT stankovalibuse uptakeandreductionofoxidizedandreducedascorbatebyhumanleukocytes

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