A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY

A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clo...

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Detalles Bibliográficos
Autores principales: Rosenstreich, David L., Asselineau, Jean, Mergenhagen, Stephan E., Nowotny, Alois
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Brief Definitive Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139720/
https://www.ncbi.nlm.nih.gov/pubmed/4547545
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author Rosenstreich, David L.
Asselineau, Jean
Mergenhagen, Stephan E.
Nowotny, Alois
author_facet Rosenstreich, David L.
Asselineau, Jean
Mergenhagen, Stephan E.
Nowotny, Alois
author_sort Rosenstreich, David L.
collection PubMed
description A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contamination, or that NPG itself is 10(–5) times less active in this assay than purified endotoxic LPS. Since such low levels of endotoxin concentration are not mitogenic, it is concluded that synthetic N-palmitoyl-D-glucosamine, when properly dispersed, is itself a B-cell mitogen.
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spelling pubmed-21397202008-04-17 A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY Rosenstreich, David L. Asselineau, Jean Mergenhagen, Stephan E. Nowotny, Alois J Exp Med Brief Definitive Reports A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contamination, or that NPG itself is 10(–5) times less active in this assay than purified endotoxic LPS. Since such low levels of endotoxin concentration are not mitogenic, it is concluded that synthetic N-palmitoyl-D-glucosamine, when properly dispersed, is itself a B-cell mitogen. The Rockefeller University Press 1974-10-31 /pmc/articles/PMC2139720/ /pubmed/4547545 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Rosenstreich, David L.
Asselineau, Jean
Mergenhagen, Stephan E.
Nowotny, Alois
A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title_full A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title_fullStr A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title_full_unstemmed A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title_short A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY
title_sort synthetic glycolipid with b-cell mitogenic activity
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139720/
https://www.ncbi.nlm.nih.gov/pubmed/4547545
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