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STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS
Rabbit synovial fibroblasts in monolayer culture secrete a specific collagenase and a neutral endopeptidase into their serum-free culture medium. The rate of secretion of these two enzymes is increased after the ingestion and storage of latex particles within the vacuolar system of the cells. The in...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1974
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139759/ https://www.ncbi.nlm.nih.gov/pubmed/4372292 |
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author | Werb, Zena Reynolds, John J. |
author_facet | Werb, Zena Reynolds, John J. |
author_sort | Werb, Zena |
collection | PubMed |
description | Rabbit synovial fibroblasts in monolayer culture secrete a specific collagenase and a neutral endopeptidase into their serum-free culture medium. The rate of secretion of these two enzymes is increased after the ingestion and storage of latex particles within the vacuolar system of the cells. The increased rates of secretion of the neutral enzymes are stable for over 2 wk in the absence of a further phagocytic bout. In constrast there is little change in the extracellular levels of two lysosomal hydrolases, cathepsin D and β-glucuronidase. The increase in the secretory rates for the two neutral enzymes is related to the number of latex particles ingested by the cells, and increases of up to 12-fold over the nonphagocytosing cultures were observed. A variety of other materials including mycostatin particles and dextran sulfate also induced increases in the secretion of collagenase. These results are discussed in relation to the turnover of connective tissue matrix macromolecules. |
format | Text |
id | pubmed-2139759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1974 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21397592008-04-17 STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS Werb, Zena Reynolds, John J. J Exp Med Article Rabbit synovial fibroblasts in monolayer culture secrete a specific collagenase and a neutral endopeptidase into their serum-free culture medium. The rate of secretion of these two enzymes is increased after the ingestion and storage of latex particles within the vacuolar system of the cells. The increased rates of secretion of the neutral enzymes are stable for over 2 wk in the absence of a further phagocytic bout. In constrast there is little change in the extracellular levels of two lysosomal hydrolases, cathepsin D and β-glucuronidase. The increase in the secretory rates for the two neutral enzymes is related to the number of latex particles ingested by the cells, and increases of up to 12-fold over the nonphagocytosing cultures were observed. A variety of other materials including mycostatin particles and dextran sulfate also induced increases in the secretion of collagenase. These results are discussed in relation to the turnover of connective tissue matrix macromolecules. The Rockefeller University Press 1974-11-30 /pmc/articles/PMC2139759/ /pubmed/4372292 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Werb, Zena Reynolds, John J. STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title | STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title_full | STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title_fullStr | STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title_full_unstemmed | STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title_short | STIMULATION BY ENDOCYTOSIS OF THE SECRETION OF COLLAGENASE AND NEUTRAL PROTEINASE FROM RABBIT SYNOVIAL FIBROBLASTS |
title_sort | stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139759/ https://www.ncbi.nlm.nih.gov/pubmed/4372292 |
work_keys_str_mv | AT werbzena stimulationbyendocytosisofthesecretionofcollagenaseandneutralproteinasefromrabbitsynovialfibroblasts AT reynoldsjohnj stimulationbyendocytosisofthesecretionofcollagenaseandneutralproteinasefromrabbitsynovialfibroblasts |