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Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction

A novel actin filament (F-actin)–binding protein with a molecular mass of ∼205 kD (p205), which was concentrated at cadherin-based cell-to-cell adherens junction (AJ), was isolated and characterized. p205 was purified from rat brain and its cDNA was cloned from a rat brain cDNA library. p205 was a p...

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Autores principales: Mandai, Kenji, Nakanishi, Hiroyuki, Satoh, Ayako, Obaishi, Hiroshi, Wada, Manabu, Nishioka, Hideo, Itoh, Masahiko, Mizoguchi, Akira, Aoki, Takeo, Fujimoto, Toyoshi, Matsuda, Yoichi, Tsukita, Shoichiro, Takai, Yoshimi
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139800/
https://www.ncbi.nlm.nih.gov/pubmed/9334353
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author Mandai, Kenji
Nakanishi, Hiroyuki
Satoh, Ayako
Obaishi, Hiroshi
Wada, Manabu
Nishioka, Hideo
Itoh, Masahiko
Mizoguchi, Akira
Aoki, Takeo
Fujimoto, Toyoshi
Matsuda, Yoichi
Tsukita, Shoichiro
Takai, Yoshimi
author_facet Mandai, Kenji
Nakanishi, Hiroyuki
Satoh, Ayako
Obaishi, Hiroshi
Wada, Manabu
Nishioka, Hideo
Itoh, Masahiko
Mizoguchi, Akira
Aoki, Takeo
Fujimoto, Toyoshi
Matsuda, Yoichi
Tsukita, Shoichiro
Takai, Yoshimi
author_sort Mandai, Kenji
collection PubMed
description A novel actin filament (F-actin)–binding protein with a molecular mass of ∼205 kD (p205), which was concentrated at cadherin-based cell-to-cell adherens junction (AJ), was isolated and characterized. p205 was purified from rat brain and its cDNA was cloned from a rat brain cDNA library. p205 was a protein of 1,829 amino acids (aa) with a calculated molecular mass of 207,667 kD. p205 had one F-actin–binding domain at 1,631–1,829 aa residues and one PDZ domain at 1,016– 1,100 aa residues, a domain known to interact with transmembrane proteins. p205 was copurified from rat brain with another protein with a molecular mass of 190 kD (p190). p190 was a protein of 1,663 aa with a calculated molecular mass of 188,971 kD. p190 was a splicing variant of p205 having one PDZ domain at 1,009–1,093 aa residues but lacking the F-actin–binding domain. Homology search analysis revealed that the aa sequence of p190 showed 90% identity over the entire sequence with the product of the AF-6 gene, which was found to be fused to the ALL-1 gene, known to be involved in acute leukemia. p190 is likely to be a rat counterpart of human AF-6 protein. p205 bound along the sides of F-actin but hardly showed the F-actin–cross-linking activity. Northern and Western blot analyses showed that p205 was ubiquitously expressed in all the rat tissues examined, whereas p190 was specifically expressed in brain. Immunofluorescence and immunoelectron microscopic studies revealed that p205 was concentrated at cadherin-based cell-to-cell AJ of various tissues. We named p205 l-afadin (a large splicing variant of AF-6 protein localized at adherens junction) and p190 s-afadin (a small splicing variant of l-afadin). These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ.
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spelling pubmed-21398002008-05-01 Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction Mandai, Kenji Nakanishi, Hiroyuki Satoh, Ayako Obaishi, Hiroshi Wada, Manabu Nishioka, Hideo Itoh, Masahiko Mizoguchi, Akira Aoki, Takeo Fujimoto, Toyoshi Matsuda, Yoichi Tsukita, Shoichiro Takai, Yoshimi J Cell Biol Article A novel actin filament (F-actin)–binding protein with a molecular mass of ∼205 kD (p205), which was concentrated at cadherin-based cell-to-cell adherens junction (AJ), was isolated and characterized. p205 was purified from rat brain and its cDNA was cloned from a rat brain cDNA library. p205 was a protein of 1,829 amino acids (aa) with a calculated molecular mass of 207,667 kD. p205 had one F-actin–binding domain at 1,631–1,829 aa residues and one PDZ domain at 1,016– 1,100 aa residues, a domain known to interact with transmembrane proteins. p205 was copurified from rat brain with another protein with a molecular mass of 190 kD (p190). p190 was a protein of 1,663 aa with a calculated molecular mass of 188,971 kD. p190 was a splicing variant of p205 having one PDZ domain at 1,009–1,093 aa residues but lacking the F-actin–binding domain. Homology search analysis revealed that the aa sequence of p190 showed 90% identity over the entire sequence with the product of the AF-6 gene, which was found to be fused to the ALL-1 gene, known to be involved in acute leukemia. p190 is likely to be a rat counterpart of human AF-6 protein. p205 bound along the sides of F-actin but hardly showed the F-actin–cross-linking activity. Northern and Western blot analyses showed that p205 was ubiquitously expressed in all the rat tissues examined, whereas p190 was specifically expressed in brain. Immunofluorescence and immunoelectron microscopic studies revealed that p205 was concentrated at cadherin-based cell-to-cell AJ of various tissues. We named p205 l-afadin (a large splicing variant of AF-6 protein localized at adherens junction) and p190 s-afadin (a small splicing variant of l-afadin). These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ. The Rockefeller University Press 1997-10-20 /pmc/articles/PMC2139800/ /pubmed/9334353 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Mandai, Kenji
Nakanishi, Hiroyuki
Satoh, Ayako
Obaishi, Hiroshi
Wada, Manabu
Nishioka, Hideo
Itoh, Masahiko
Mizoguchi, Akira
Aoki, Takeo
Fujimoto, Toyoshi
Matsuda, Yoichi
Tsukita, Shoichiro
Takai, Yoshimi
Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title_full Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title_fullStr Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title_full_unstemmed Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title_short Afadin: A Novel Actin Filament–binding Protein with One PDZ Domain Localized at Cadherin-based Cell-to-Cell Adherens Junction
title_sort afadin: a novel actin filament–binding protein with one pdz domain localized at cadherin-based cell-to-cell adherens junction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139800/
https://www.ncbi.nlm.nih.gov/pubmed/9334353
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