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An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids

Basonuclin is a zinc finger protein that was thought to be restricted to keratinocytes of stratified squamous epithelia. In epidermis, basonuclin is associated with the nuclei of mitotically active basal cells but not in terminally differentiating keratinocytes. We report here the isolation of a nov...

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Detalles Bibliográficos
Autores principales: Yang, Zhao-hui, Gallicano, G. Ian, Yu, Qian-Chun, Fuchs, Elaine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139879/
https://www.ncbi.nlm.nih.gov/pubmed/9151672
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author Yang, Zhao-hui
Gallicano, G. Ian
Yu, Qian-Chun
Fuchs, Elaine
author_facet Yang, Zhao-hui
Gallicano, G. Ian
Yu, Qian-Chun
Fuchs, Elaine
author_sort Yang, Zhao-hui
collection PubMed
description Basonuclin is a zinc finger protein that was thought to be restricted to keratinocytes of stratified squamous epithelia. In epidermis, basonuclin is associated with the nuclei of mitotically active basal cells but not in terminally differentiating keratinocytes. We report here the isolation of a novel form of basonuclin, which we show is also expressed in stratified epithelia. Most unexpectedly, we find both forms in testis, where a surprising localization pattern was uncovered. While basonuclin RNA expression occurs in mitotically active germ cells, protein was not detected until the meiotic stage, where basonuclin localized to the appendage of the distal centriole of spermatocytes and spermatids. Near the end of spermiogenesis, basonuclin also accumulated in the acrosome and mitochondrial sheath surrounding the flagellum. Intriguingly, a perfect six– amino acid residue mitochondrial targeting sequence (Komiya, T., N. Hachiya, M. Sakaguchi, T. Omura, and K. Mihara. 1994. J. Biol. Chem. 269:30893–30897; Shore, G.C., H.M. McBride, D.G. Millar, N.A. Steenaart, and M. Nguyen. 1995. Eur. J. Biochem. 227: 9–18; McBride, H.M., I.S. Goping, and G.C. Shore. 1996. J. Cell. Biol. 134:307–313) is present in basonuclin 1a but not in the 1b form. Moreover, three distinct affinity-purified peptide antibodies gave this unusual pattern of basonuclin antibody staining, which was confirmed by cell fractionation studies. Our findings suggest a unique role for basonuclin in centrosomes within the developing spermatid, and a role for one of the protein forms in germ cell mitochondrial function. Its localization with the acrosome suggests that it may also perform a special function during or shortly after fertilization.
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spelling pubmed-21398792008-05-01 An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids Yang, Zhao-hui Gallicano, G. Ian Yu, Qian-Chun Fuchs, Elaine J Cell Biol Article Basonuclin is a zinc finger protein that was thought to be restricted to keratinocytes of stratified squamous epithelia. In epidermis, basonuclin is associated with the nuclei of mitotically active basal cells but not in terminally differentiating keratinocytes. We report here the isolation of a novel form of basonuclin, which we show is also expressed in stratified epithelia. Most unexpectedly, we find both forms in testis, where a surprising localization pattern was uncovered. While basonuclin RNA expression occurs in mitotically active germ cells, protein was not detected until the meiotic stage, where basonuclin localized to the appendage of the distal centriole of spermatocytes and spermatids. Near the end of spermiogenesis, basonuclin also accumulated in the acrosome and mitochondrial sheath surrounding the flagellum. Intriguingly, a perfect six– amino acid residue mitochondrial targeting sequence (Komiya, T., N. Hachiya, M. Sakaguchi, T. Omura, and K. Mihara. 1994. J. Biol. Chem. 269:30893–30897; Shore, G.C., H.M. McBride, D.G. Millar, N.A. Steenaart, and M. Nguyen. 1995. Eur. J. Biochem. 227: 9–18; McBride, H.M., I.S. Goping, and G.C. Shore. 1996. J. Cell. Biol. 134:307–313) is present in basonuclin 1a but not in the 1b form. Moreover, three distinct affinity-purified peptide antibodies gave this unusual pattern of basonuclin antibody staining, which was confirmed by cell fractionation studies. Our findings suggest a unique role for basonuclin in centrosomes within the developing spermatid, and a role for one of the protein forms in germ cell mitochondrial function. Its localization with the acrosome suggests that it may also perform a special function during or shortly after fertilization. The Rockefeller University Press 1997-05-05 /pmc/articles/PMC2139879/ /pubmed/9151672 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Yang, Zhao-hui
Gallicano, G. Ian
Yu, Qian-Chun
Fuchs, Elaine
An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title_full An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title_fullStr An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title_full_unstemmed An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title_short An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids
title_sort unexpected localization of basonuclin in the centrosome, mitochondria, and acrosome of developing spermatids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139879/
https://www.ncbi.nlm.nih.gov/pubmed/9151672
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