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Neocentromere-mediated Chromosome Movement in Maize
Neocentromere activity is a classic example of nonkinetochore chromosome movement. In maize, neocentromeres are induced by a gene or genes on Abnormal chromosome 10 (Ab10) which causes heterochromatic knobs to move poleward at meiotic anaphase. Here we describe experiments that test how neocentromer...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139958/ https://www.ncbi.nlm.nih.gov/pubmed/9362502 |
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author | Yu, Hong-Guo Hiatt, Evelyn N. Chan, Annette Sweeney, Mary Dawe, R. Kelly |
author_facet | Yu, Hong-Guo Hiatt, Evelyn N. Chan, Annette Sweeney, Mary Dawe, R. Kelly |
author_sort | Yu, Hong-Guo |
collection | PubMed |
description | Neocentromere activity is a classic example of nonkinetochore chromosome movement. In maize, neocentromeres are induced by a gene or genes on Abnormal chromosome 10 (Ab10) which causes heterochromatic knobs to move poleward at meiotic anaphase. Here we describe experiments that test how neocentromere activity affects the function of linked centromere/kinetochores (kinetochores) and whether neocentromeres and kinetochores are mobilized on the spindle by the same mechanism. Using a newly developed system for observing meiotic chromosome congression and segregation in living maize cells, we show that neocentromeres are active from prometaphase through anaphase. During mid-anaphase, normal chromosomes move on the spindle at an average rate of 0.79 μm/min. The presence of Ab10 does not affect the rate of normal chromosome movement but propels neocentromeres poleward at rates as high as 1.4 μm/min. Kinetochore-mediated chromosome movement is only marginally affected by the activity of a linked neocentromere. Combined in situ hybridization/immunocytochemistry is used to demonstrate that unlike kinetochores, neocentromeres associate laterally with microtubules and that neocentromere movement is correlated with knob size. These data suggest that microtubule depolymerization is not required for neocentromere motility. We argue that neocentromeres are mobilized on microtubules by the activity of minus end–directed motor proteins that interact either directly or indirectly with knob DNA sequences. |
format | Text |
id | pubmed-2139958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21399582008-05-01 Neocentromere-mediated Chromosome Movement in Maize Yu, Hong-Guo Hiatt, Evelyn N. Chan, Annette Sweeney, Mary Dawe, R. Kelly J Cell Biol Article Neocentromere activity is a classic example of nonkinetochore chromosome movement. In maize, neocentromeres are induced by a gene or genes on Abnormal chromosome 10 (Ab10) which causes heterochromatic knobs to move poleward at meiotic anaphase. Here we describe experiments that test how neocentromere activity affects the function of linked centromere/kinetochores (kinetochores) and whether neocentromeres and kinetochores are mobilized on the spindle by the same mechanism. Using a newly developed system for observing meiotic chromosome congression and segregation in living maize cells, we show that neocentromeres are active from prometaphase through anaphase. During mid-anaphase, normal chromosomes move on the spindle at an average rate of 0.79 μm/min. The presence of Ab10 does not affect the rate of normal chromosome movement but propels neocentromeres poleward at rates as high as 1.4 μm/min. Kinetochore-mediated chromosome movement is only marginally affected by the activity of a linked neocentromere. Combined in situ hybridization/immunocytochemistry is used to demonstrate that unlike kinetochores, neocentromeres associate laterally with microtubules and that neocentromere movement is correlated with knob size. These data suggest that microtubule depolymerization is not required for neocentromere motility. We argue that neocentromeres are mobilized on microtubules by the activity of minus end–directed motor proteins that interact either directly or indirectly with knob DNA sequences. The Rockefeller University Press 1997-11-17 /pmc/articles/PMC2139958/ /pubmed/9362502 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Yu, Hong-Guo Hiatt, Evelyn N. Chan, Annette Sweeney, Mary Dawe, R. Kelly Neocentromere-mediated Chromosome Movement in Maize |
title | Neocentromere-mediated Chromosome Movement in Maize |
title_full | Neocentromere-mediated Chromosome Movement in Maize |
title_fullStr | Neocentromere-mediated Chromosome Movement in Maize |
title_full_unstemmed | Neocentromere-mediated Chromosome Movement in Maize |
title_short | Neocentromere-mediated Chromosome Movement in Maize |
title_sort | neocentromere-mediated chromosome movement in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139958/ https://www.ncbi.nlm.nih.gov/pubmed/9362502 |
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