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RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO
BACKGROUND: The Raf kinase inhibitor protein (RKIP) has been shown to block MAP kinase pathway as well as NFκB signalling. By means of immunohistochemistry, we previously demonstrated that the MAP kinase pathway is virtually inactive in Merkel cell carcinoma (MCC). Similarly to MCC in situ high RKIP...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140056/ https://www.ncbi.nlm.nih.gov/pubmed/17958888 http://dx.doi.org/10.1186/1477-3163-6-16 |
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author | Houben, Roland Ortmann, Sonja Becker, Juergen C |
author_facet | Houben, Roland Ortmann, Sonja Becker, Juergen C |
author_sort | Houben, Roland |
collection | PubMed |
description | BACKGROUND: The Raf kinase inhibitor protein (RKIP) has been shown to block MAP kinase pathway as well as NFκB signalling. By means of immunohistochemistry, we previously demonstrated that the MAP kinase pathway is virtually inactive in Merkel cell carcinoma (MCC). Similarly to MCC in situ high RKIP expression accompanies absence of ERK phosphorylation in the MCC cell line UISO suggesting that RKIP might be causative for MAP kinase pathway silencing. METHODS: Applying an siRNA approach RKIP expression was knocked down in UISO cells and a possible influence on MAP kinase pathway activity was assessed by Western blot analysis using phospho-specific antibodies. Moreover, a possible effect of RKIP knock down in UISO cells on proliferation as well as chemosensitivity to cisplatin were examined applying the MTS assay. RESULTS: Surprisingly the absence of phosphorylation of the MAP kinases ERK1 and ERK 2 even following growth factor stimulation was not affected by the RKIP knock down indicating that RKIP is not essential for blocking the MAP kinase pathway in the MCC cell line UISO. Moreover, proliferation as well as chemosensitivity towards cisplatin were not altered upon knock down of RKIP. |
format | Text |
id | pubmed-2140056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-21400562007-12-15 RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO Houben, Roland Ortmann, Sonja Becker, Juergen C J Carcinog Short Paper BACKGROUND: The Raf kinase inhibitor protein (RKIP) has been shown to block MAP kinase pathway as well as NFκB signalling. By means of immunohistochemistry, we previously demonstrated that the MAP kinase pathway is virtually inactive in Merkel cell carcinoma (MCC). Similarly to MCC in situ high RKIP expression accompanies absence of ERK phosphorylation in the MCC cell line UISO suggesting that RKIP might be causative for MAP kinase pathway silencing. METHODS: Applying an siRNA approach RKIP expression was knocked down in UISO cells and a possible influence on MAP kinase pathway activity was assessed by Western blot analysis using phospho-specific antibodies. Moreover, a possible effect of RKIP knock down in UISO cells on proliferation as well as chemosensitivity to cisplatin were examined applying the MTS assay. RESULTS: Surprisingly the absence of phosphorylation of the MAP kinases ERK1 and ERK 2 even following growth factor stimulation was not affected by the RKIP knock down indicating that RKIP is not essential for blocking the MAP kinase pathway in the MCC cell line UISO. Moreover, proliferation as well as chemosensitivity towards cisplatin were not altered upon knock down of RKIP. BioMed Central 2007-10-24 /pmc/articles/PMC2140056/ /pubmed/17958888 http://dx.doi.org/10.1186/1477-3163-6-16 Text en Copyright © 2007 Houben et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Paper Houben, Roland Ortmann, Sonja Becker, Juergen C RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title | RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title_full | RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title_fullStr | RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title_full_unstemmed | RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title_short | RKIP does not contribute to MAP kinase pathway silencing in the Merkel Cell Carcinoma cell line UISO |
title_sort | rkip does not contribute to map kinase pathway silencing in the merkel cell carcinoma cell line uiso |
topic | Short Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140056/ https://www.ncbi.nlm.nih.gov/pubmed/17958888 http://dx.doi.org/10.1186/1477-3163-6-16 |
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