Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method

Genotype 4 hepatitis E virus (HEV) was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate (SH-SW-zs1) recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR...

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Autores principales: Shen, Quan, Zhang, Wen, Cao, Xiangrong, Mou, Jing, Cui, Li, Hua, Xiuguo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140057/
https://www.ncbi.nlm.nih.gov/pubmed/17925017
http://dx.doi.org/10.1186/1743-422X-4-98
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author Shen, Quan
Zhang, Wen
Cao, Xiangrong
Mou, Jing
Cui, Li
Hua, Xiuguo
author_facet Shen, Quan
Zhang, Wen
Cao, Xiangrong
Mou, Jing
Cui, Li
Hua, Xiuguo
author_sort Shen, Quan
collection PubMed
description Genotype 4 hepatitis E virus (HEV) was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate (SH-SW-zs1) recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR and RACE (Rapid Amplification of cDNA Ends) methods. The full genome of the SH-SW-zs1 isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype 4. Comparison of the 3' UTR sequence with the corresponding regions of other 38 HEV strains from different region revealed that the Shanghai swine isolate is 21–49 bp longer than the other stains.
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spelling pubmed-21400572007-12-15 Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method Shen, Quan Zhang, Wen Cao, Xiangrong Mou, Jing Cui, Li Hua, Xiuguo Virol J Research Genotype 4 hepatitis E virus (HEV) was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate (SH-SW-zs1) recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR and RACE (Rapid Amplification of cDNA Ends) methods. The full genome of the SH-SW-zs1 isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype 4. Comparison of the 3' UTR sequence with the corresponding regions of other 38 HEV strains from different region revealed that the Shanghai swine isolate is 21–49 bp longer than the other stains. BioMed Central 2007-10-09 /pmc/articles/PMC2140057/ /pubmed/17925017 http://dx.doi.org/10.1186/1743-422X-4-98 Text en Copyright © 2007 Shen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Shen, Quan
Zhang, Wen
Cao, Xiangrong
Mou, Jing
Cui, Li
Hua, Xiuguo
Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title_full Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title_fullStr Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title_full_unstemmed Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title_short Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
title_sort cloning of full genome sequence of hepatitis e virus of shanghai swine isolate using race method
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140057/
https://www.ncbi.nlm.nih.gov/pubmed/17925017
http://dx.doi.org/10.1186/1743-422X-4-98
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