DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY

1. Methods are described for obtaining cell sap from Nitella without contamination. 2. Tests of the cell sap show that in a balanced solution of NaNO(3) plus Ca(NO(3))(2) there is a slow penetration of NO(3) and that the cell remains in a normal condition, but in pure NaNO(3) there is rapid penetrat...

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Detalles Bibliográficos
Autor principal: Osterhout, W. J. V.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1922
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140504/
https://www.ncbi.nlm.nih.gov/pubmed/19871931
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author Osterhout, W. J. V.
author_facet Osterhout, W. J. V.
author_sort Osterhout, W. J. V.
collection PubMed
description 1. Methods are described for obtaining cell sap from Nitella without contamination. 2. Tests of the cell sap show that in a balanced solution of NaNO(3) plus Ca(NO(3))(2) there is a slow penetration of NO(3) and that the cell remains in a normal condition, but in pure NaNO(3) there is rapid penetration accompanied by injury. 3. Inasmuch as determinations of electrical conductivity give the same result it may be concluded that this method gives reliable information regarding permeability. 4. While observations on recovery from plasmolysis give similar results, the method is less satisfactory.
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spelling pubmed-21405042008-04-23 DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY Osterhout, W. J. V. J Gen Physiol Article 1. Methods are described for obtaining cell sap from Nitella without contamination. 2. Tests of the cell sap show that in a balanced solution of NaNO(3) plus Ca(NO(3))(2) there is a slow penetration of NO(3) and that the cell remains in a normal condition, but in pure NaNO(3) there is rapid penetration accompanied by injury. 3. Inasmuch as determinations of electrical conductivity give the same result it may be concluded that this method gives reliable information regarding permeability. 4. While observations on recovery from plasmolysis give similar results, the method is less satisfactory. The Rockefeller University Press 1922-01-20 /pmc/articles/PMC2140504/ /pubmed/19871931 Text en Copyright © Copyright, 1922, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Osterhout, W. J. V.
DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title_full DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title_fullStr DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title_full_unstemmed DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title_short DIRECT AND INDIRECT DETERMINATIONS OF PERMEABILITY
title_sort direct and indirect determinations of permeability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140504/
https://www.ncbi.nlm.nih.gov/pubmed/19871931
work_keys_str_mv AT osterhoutwjv directandindirectdeterminationsofpermeability

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