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THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G

The acid agglutination optimum of Microbes D and G is not independent of the nature of the buffer mixture. Glycocoll-HCl buffer mixtures cause complete flocculation at high C(H)+ (2.7 to 2.4), at which points little or no flocculation occurs with the Na lactate-lactic acid buffer series. Beef infusi...

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Autor principal: De Kruif, Paul H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1922
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140506/
https://www.ncbi.nlm.nih.gov/pubmed/19871943
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author De Kruif, Paul H.
author_facet De Kruif, Paul H.
author_sort De Kruif, Paul H.
collection PubMed
description The acid agglutination optimum of Microbes D and G is not independent of the nature of the buffer mixture. Glycocoll-HCl buffer mixtures cause complete flocculation at high C(H)+ (2.7 to 2.4), at which points little or no flocculation occurs with the Na lactate-lactic acid buffer series. Beef infusion has the property of broadening the acid agglutination optimum of both Microbes D and G, bacilli of rabbit septicemia. This extension is in the direction of a lower C(H)+. There is no evidence that the beef infusion has the power, per se, of agglutinating these organisms. It would seem merely to increase their sensitiveness to sedimentation in the presence of H ions. The data presented explain the mechanism of the granular growth character of Microbe G in liquid media as compared to the diffuse growth of Microbe D. Peptone (Fairchild), contrary to beef infusion, shifts the acid agglutination optimum of Microbes D and G in the direction of a higher C(H)+. Strong concentrations of peptone exhibit an inhibitory effect on the agglutination of Microbe D in the optimum zone.
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spelling pubmed-21405062008-04-23 THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G De Kruif, Paul H. J Gen Physiol Article The acid agglutination optimum of Microbes D and G is not independent of the nature of the buffer mixture. Glycocoll-HCl buffer mixtures cause complete flocculation at high C(H)+ (2.7 to 2.4), at which points little or no flocculation occurs with the Na lactate-lactic acid buffer series. Beef infusion has the property of broadening the acid agglutination optimum of both Microbes D and G, bacilli of rabbit septicemia. This extension is in the direction of a lower C(H)+. There is no evidence that the beef infusion has the power, per se, of agglutinating these organisms. It would seem merely to increase their sensitiveness to sedimentation in the presence of H ions. The data presented explain the mechanism of the granular growth character of Microbe G in liquid media as compared to the diffuse growth of Microbe D. Peptone (Fairchild), contrary to beef infusion, shifts the acid agglutination optimum of Microbes D and G in the direction of a higher C(H)+. Strong concentrations of peptone exhibit an inhibitory effect on the agglutination of Microbe D in the optimum zone. The Rockefeller University Press 1922-03-20 /pmc/articles/PMC2140506/ /pubmed/19871943 Text en Copyright © Copyright, 1922, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
De Kruif, Paul H.
THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title_full THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title_fullStr THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title_full_unstemmed THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title_short THE MECHANISM OF GRANULAR GROWTH OF RABBIT SEPTICEMIA BACILLUS TYPE G
title_sort mechanism of granular growth of rabbit septicemia bacillus type g
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140506/
https://www.ncbi.nlm.nih.gov/pubmed/19871943
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