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A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN

A quantitative method is described which permits a determination of the relative amount of trypsin or pepsin present in a gelatin-enzyme digestion mixture, provided the gelatin and trypsin solutions are purified. This method is dependent upon the change in viscosity of such solutions. It is found th...

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Detalles Bibliográficos
Autores principales: Northrop, John H., Hussey, Raymond G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1923
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140532/
https://www.ncbi.nlm.nih.gov/pubmed/19872001
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author Northrop, John H.
Hussey, Raymond G.
author_facet Northrop, John H.
Hussey, Raymond G.
author_sort Northrop, John H.
collection PubMed
description A quantitative method is described which permits a determination of the relative amount of trypsin or pepsin present in a gelatin-enzyme digestion mixture, provided the gelatin and trypsin solutions are purified. This method is dependent upon the change in viscosity of such solutions. It is found that the time required to cause a given percentage change in the viscosity is nearly inversely proportional to the amount of enzyme present. It is pointed out that the particular value of the method lies in the fact that enzyme reactions which take place in the presence of "buffer" salts may be studied.
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spelling pubmed-21405322008-04-23 A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN Northrop, John H. Hussey, Raymond G. J Gen Physiol Article A quantitative method is described which permits a determination of the relative amount of trypsin or pepsin present in a gelatin-enzyme digestion mixture, provided the gelatin and trypsin solutions are purified. This method is dependent upon the change in viscosity of such solutions. It is found that the time required to cause a given percentage change in the viscosity is nearly inversely proportional to the amount of enzyme present. It is pointed out that the particular value of the method lies in the fact that enzyme reactions which take place in the presence of "buffer" salts may be studied. The Rockefeller University Press 1923-01-20 /pmc/articles/PMC2140532/ /pubmed/19872001 Text en Copyright © Copyright, 1923, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Northrop, John H.
Hussey, Raymond G.
A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title_full A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title_fullStr A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title_full_unstemmed A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title_short A METHOD FOR THE QUANTITATIVE DETERMINATION OF TRYPSIN AND PEPSIN
title_sort method for the quantitative determination of trypsin and pepsin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140532/
https://www.ncbi.nlm.nih.gov/pubmed/19872001
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