THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL

Irregularities in migration velocity of bacterial cells in the highly alkaline solutions are due to the buffering effect of the cells upon the immediately adjacent zone of menstruum. Consistent results can be obtained by shaking the suspension thoroughly before placing it in the electrophoretic cell...

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Detalles Bibliográficos
Autores principales: Winslow, C.-E. A., Shaughnessy, H. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1924
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140689/
https://www.ncbi.nlm.nih.gov/pubmed/19872109
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author Winslow, C.-E. A.
Shaughnessy, H. J.
author_facet Winslow, C.-E. A.
Shaughnessy, H. J.
author_sort Winslow, C.-E. A.
collection PubMed
description Irregularities in migration velocity of bacterial cells in the highly alkaline solutions are due to the buffering effect of the cells upon the immediately adjacent zone of menstruum. Consistent results can be obtained by shaking the suspension thoroughly before placing it in the electrophoretic cell. When observed in this way both Bacillus cereus and Bacterium coli show an isopotential point near pH 13.5, that for Bacillus cereus being slightly below, and that for Bacterium coli slightly above this point. At more alkaline reactions the cells acquire a positive charge which increases with further increase in pH to very high values.
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spelling pubmed-21406892008-04-23 THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL Winslow, C.-E. A. Shaughnessy, H. J. J Gen Physiol Article Irregularities in migration velocity of bacterial cells in the highly alkaline solutions are due to the buffering effect of the cells upon the immediately adjacent zone of menstruum. Consistent results can be obtained by shaking the suspension thoroughly before placing it in the electrophoretic cell. When observed in this way both Bacillus cereus and Bacterium coli show an isopotential point near pH 13.5, that for Bacillus cereus being slightly below, and that for Bacterium coli slightly above this point. At more alkaline reactions the cells acquire a positive charge which increases with further increase in pH to very high values. The Rockefeller University Press 1924-07-20 /pmc/articles/PMC2140689/ /pubmed/19872109 Text en Copyright © Copyright, 1924, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Winslow, C.-E. A.
Shaughnessy, H. J.
THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title_full THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title_fullStr THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title_full_unstemmed THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title_short THE ALKALINE ISOPOTENTIAL POINT OF THE BACTERIAL CELL
title_sort alkaline isopotential point of the bacterial cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140689/
https://www.ncbi.nlm.nih.gov/pubmed/19872109
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