THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS

The rates of digestion of keratose have been determined with three commercial enzymes, ranging widely in strength. It has been found that the weaker the enzyme preparation, the more nearly does the course of the hydrolysis conform to that of a reaction of the first order. This has been explained on...

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Detalles Bibliográficos
Autor principal: Merrill, Henry Baldwin
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1926
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140890/
https://www.ncbi.nlm.nih.gov/pubmed/19872317
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author Merrill, Henry Baldwin
author_facet Merrill, Henry Baldwin
author_sort Merrill, Henry Baldwin
collection PubMed
description The rates of digestion of keratose have been determined with three commercial enzymes, ranging widely in strength. It has been found that the weaker the enzyme preparation, the more nearly does the course of the hydrolysis conform to that of a reaction of the first order. This has been explained on the assumption that in solution an equilibrium exists between active enzyme, and enzyme combined with inert material. In very impure enzyme preparations, the large quantities of combined enzyme act as a reservoir for active enzyme, maintaining a constant concentration of active enzyme during the course of the digestion.
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spelling pubmed-21408902008-04-23 THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS Merrill, Henry Baldwin J Gen Physiol Article The rates of digestion of keratose have been determined with three commercial enzymes, ranging widely in strength. It has been found that the weaker the enzyme preparation, the more nearly does the course of the hydrolysis conform to that of a reaction of the first order. This has been explained on the assumption that in solution an equilibrium exists between active enzyme, and enzyme combined with inert material. In very impure enzyme preparations, the large quantities of combined enzyme act as a reservoir for active enzyme, maintaining a constant concentration of active enzyme during the course of the digestion. The Rockefeller University Press 1926-11-20 /pmc/articles/PMC2140890/ /pubmed/19872317 Text en Copyright © Copyright, 1926, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Merrill, Henry Baldwin
THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title_full THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title_fullStr THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title_full_unstemmed THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title_short THE EFFECT OF ENZYME PURITY ON THE KINETICS OF TRYPTIC HYDROLYSIS
title_sort effect of enzyme purity on the kinetics of tryptic hydrolysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2140890/
https://www.ncbi.nlm.nih.gov/pubmed/19872317
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