A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL

1. A method for measuring the O(2) consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas,...

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Detalles Bibliográficos
Autores principales: Howland, Ruth B., Bernstein, Alan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1931
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141112/
https://www.ncbi.nlm.nih.gov/pubmed/19872589
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author Howland, Ruth B.
Bernstein, Alan
author_facet Howland, Ruth B.
Bernstein, Alan
author_sort Howland, Ruth B.
collection PubMed
description 1. A method for measuring the O(2) consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas, representing the O(2) consumed, is followed. 2. The possible errors of the technique are appraised. 3. A single Actinosphaerium eichhornii consumes 0.00113 mm.(3) of O(2) per hour. A single Paramecium caudatum consumes 0.00049 mm.(3) of O(2) per hour. 4. The significance of the results and the limitations of the method are discussed.
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spelling pubmed-21411122008-04-23 A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL Howland, Ruth B. Bernstein, Alan J Gen Physiol Article 1. A method for measuring the O(2) consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas, representing the O(2) consumed, is followed. 2. The possible errors of the technique are appraised. 3. A single Actinosphaerium eichhornii consumes 0.00113 mm.(3) of O(2) per hour. A single Paramecium caudatum consumes 0.00049 mm.(3) of O(2) per hour. 4. The significance of the results and the limitations of the method are discussed. The Rockefeller University Press 1931-01-20 /pmc/articles/PMC2141112/ /pubmed/19872589 Text en Copyright © Copyright, 1931, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Howland, Ruth B.
Bernstein, Alan
A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title_full A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title_fullStr A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title_full_unstemmed A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title_short A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL
title_sort method for determining the oxygen consumption of a single cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141112/
https://www.ncbi.nlm.nih.gov/pubmed/19872589
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