THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS

1. The effect of phosphate on the oxidation of glyceric aldehyde by methylene blue, 1-naphthol 2-sulfonate indophenol, and phenol-indophenol has been studied. 2. At pH 4.77 in a phthalate-buffered medium phosphate does not catalyze the reaction. 3. At pH 7.9 in solutions buffered with borate, carbon...

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Detalles Bibliográficos
Autores principales: Barmore, Mark, Luck, James Murray
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1931
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141146/
https://www.ncbi.nlm.nih.gov/pubmed/19872630
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author Barmore, Mark
Luck, James Murray
author_facet Barmore, Mark
Luck, James Murray
author_sort Barmore, Mark
collection PubMed
description 1. The effect of phosphate on the oxidation of glyceric aldehyde by methylene blue, 1-naphthol 2-sulfonate indophenol, and phenol-indophenol has been studied. 2. At pH 4.77 in a phthalate-buffered medium phosphate does not catalyze the reaction. 3. At pH 7.9 in solutions buffered with borate, carbonate, or phenylalanine marked catalysis by phosphate is observed. The effect is most pronounced in borate. 4. Phosphate catalysis, within the limits studied, is strictly a linear function of the phosphate concentration. 5. The high concentration of HPO(4) (=) and the low concentration of PO(4) (≡) relative to that of the substrate virtually demand the conclusion that the PO(4) (≡) ion is the active catalytic species.
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spelling pubmed-21411462008-04-23 THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS Barmore, Mark Luck, James Murray J Gen Physiol Article 1. The effect of phosphate on the oxidation of glyceric aldehyde by methylene blue, 1-naphthol 2-sulfonate indophenol, and phenol-indophenol has been studied. 2. At pH 4.77 in a phthalate-buffered medium phosphate does not catalyze the reaction. 3. At pH 7.9 in solutions buffered with borate, carbonate, or phenylalanine marked catalysis by phosphate is observed. The effect is most pronounced in borate. 4. Phosphate catalysis, within the limits studied, is strictly a linear function of the phosphate concentration. 5. The high concentration of HPO(4) (=) and the low concentration of PO(4) (≡) relative to that of the substrate virtually demand the conclusion that the PO(4) (≡) ion is the active catalytic species. The Rockefeller University Press 1931-09-20 /pmc/articles/PMC2141146/ /pubmed/19872630 Text en Copyright © Copyright, 1931, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Barmore, Mark
Luck, James Murray
THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title_full THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title_fullStr THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title_full_unstemmed THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title_short THE RÔLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
title_sort rôle of phosphate in biological oxidations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141146/
https://www.ncbi.nlm.nih.gov/pubmed/19872630
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