THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN

Inactive denatured trypsin changes into active native trypsin in the protein solutions which have been used to estimate tryptic activity. If the digestion mixture, however, is alkaline enough and contains enough urea this change does not take place. Such a digestion mixture can be used to estimate a...

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Detalles Bibliográficos
Autores principales: Anson, M. L., Mirsky, A. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1933
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141277/
https://www.ncbi.nlm.nih.gov/pubmed/19872772
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author Anson, M. L.
Mirsky, A. E.
author_facet Anson, M. L.
Mirsky, A. E.
author_sort Anson, M. L.
collection PubMed
description Inactive denatured trypsin changes into active native trypsin in the protein solutions which have been used to estimate tryptic activity. If the digestion mixture, however, is alkaline enough and contains enough urea this change does not take place. Such a digestion mixture can be used to estimate active native trypsin in the presence of inactive denatured trypsin.
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spelling pubmed-21412772008-04-23 THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN Anson, M. L. Mirsky, A. E. J Gen Physiol Article Inactive denatured trypsin changes into active native trypsin in the protein solutions which have been used to estimate tryptic activity. If the digestion mixture, however, is alkaline enough and contains enough urea this change does not take place. Such a digestion mixture can be used to estimate active native trypsin in the presence of inactive denatured trypsin. The Rockefeller University Press 1933-11-20 /pmc/articles/PMC2141277/ /pubmed/19872772 Text en Copyright © Copyright, 1933, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Anson, M. L.
Mirsky, A. E.
THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title_full THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title_fullStr THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title_full_unstemmed THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title_short THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
title_sort estimation of active native trypsin in the presence of inactive denatured trypsin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141277/
https://www.ncbi.nlm.nih.gov/pubmed/19872772
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