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A METHOD FOR DETERMINING THE RENNET ACTIVITY OF CHYMO-TRYPSIN

The rennet activity of chymo-trypsin (or pepsin) is conveniently measured by allowing a standard solution of milk to which chymotrypsin has been added to flow slowly through a graduated pipette and observing the rate and distance of flow of the milk before it clots. The time required for chymo-tryps...

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Detalles Bibliográficos
Autor principal: Kunitz, M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1935
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141362/
https://www.ncbi.nlm.nih.gov/pubmed/19872857
Descripción
Sumario:The rennet activity of chymo-trypsin (or pepsin) is conveniently measured by allowing a standard solution of milk to which chymotrypsin has been added to flow slowly through a graduated pipette and observing the rate and distance of flow of the milk before it clots. The time required for chymo-trypsin to clot milk may be calculated from these observations. The rennet activity is expressed as the reciprocal of the time in minutes required for 1 ml. of enzyme solution to clot 10 ml. of standard milk powder solution.