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ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES
1. No significant differences were noted between the electrophoretic mobilities of unground cholesterol crystallized from alcohol, acetone, or the melted state. 2. On grinding with ice at –10°C. the mobility drops to less than half (at pH 5.8) that observed in the unground state. This equilibrium co...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1935
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141414/ https://www.ncbi.nlm.nih.gov/pubmed/19872917 |
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author | Moyer, Laurence S. |
author_facet | Moyer, Laurence S. |
author_sort | Moyer, Laurence S. |
collection | PubMed |
description | 1. No significant differences were noted between the electrophoretic mobilities of unground cholesterol crystallized from alcohol, acetone, or the melted state. 2. On grinding with ice at –10°C. the mobility drops to less than half (at pH 5.8) that observed in the unground state. This equilibrium condition is reached after 50 minutes grinding. Cholesterol crystals from alcohol or acetone behave identically throughout the course of this change. 3. When cholesterol crystals which have been ground with ice are dried in vacuo at room temperature they revert to the mobility of unground cholesterol. The cause of this phenomenon is discussed. 4. Both ground and unground cholesterol have an isoelectric point near pH 3.0, even after crystallization from the melted state. |
format | Text |
id | pubmed-2141414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1935 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21414142008-04-23 ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES Moyer, Laurence S. J Gen Physiol Article 1. No significant differences were noted between the electrophoretic mobilities of unground cholesterol crystallized from alcohol, acetone, or the melted state. 2. On grinding with ice at –10°C. the mobility drops to less than half (at pH 5.8) that observed in the unground state. This equilibrium condition is reached after 50 minutes grinding. Cholesterol crystals from alcohol or acetone behave identically throughout the course of this change. 3. When cholesterol crystals which have been ground with ice are dried in vacuo at room temperature they revert to the mobility of unground cholesterol. The cause of this phenomenon is discussed. 4. Both ground and unground cholesterol have an isoelectric point near pH 3.0, even after crystallization from the melted state. The Rockefeller University Press 1935-09-20 /pmc/articles/PMC2141414/ /pubmed/19872917 Text en Copyright © Copyright, 1935, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Moyer, Laurence S. ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title | ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title_full | ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title_fullStr | ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title_full_unstemmed | ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title_short | ELECTROPHORESIS OF STEROLS : III. FURTHER INVESTIGATIONS OF CHOLESTEROL SURFACES |
title_sort | electrophoresis of sterols : iii. further investigations of cholesterol surfaces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141414/ https://www.ncbi.nlm.nih.gov/pubmed/19872917 |
work_keys_str_mv | AT moyerlaurences electrophoresisofsterolsiiifurtherinvestigationsofcholesterolsurfaces |