Cargando…

Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells

Abstract. We have studied the biosynthesis and transport of the endogenous caveolins in MDCK cells. We show that in addition to homooligomers of caveolin-1, heterooligomeric complexes of caveolin-1 and -2 are formed in the ER. The oligomers become larger, increasingly detergent insoluble, and phosph...

Descripción completa

Detalles Bibliográficos
Autores principales: Scheiffele, P., Verkade, P., Fra, A.M., Virta, H., Simons, K., Ikonen, E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141752/
https://www.ncbi.nlm.nih.gov/pubmed/9472032
_version_ 1782144251301199872
author Scheiffele, P.
Verkade, P.
Fra, A.M.
Virta, H.
Simons, K.
Ikonen, E.
author_facet Scheiffele, P.
Verkade, P.
Fra, A.M.
Virta, H.
Simons, K.
Ikonen, E.
author_sort Scheiffele, P.
collection PubMed
description Abstract. We have studied the biosynthesis and transport of the endogenous caveolins in MDCK cells. We show that in addition to homooligomers of caveolin-1, heterooligomeric complexes of caveolin-1 and -2 are formed in the ER. The oligomers become larger, increasingly detergent insoluble, and phosphorylated on caveolin-2 during transport to the cell surface. In the TGN caveolin-1/-2 heterooligomers are sorted into basolateral vesicles, whereas larger caveolin-1 homooligomers are targeted to the apical side. Caveolin-1 is present on both the apical and basolateral plasma membrane, whereas caveolin-2 is enriched on the basolateral surface where caveolae are present. This suggests that caveolin-1 and -2 heterooligomers are involved in caveolar biogenesis in the basolateral plasma membrane. Anti–caveolin-1 antibodies inhibit the apical delivery of influenza virus hemagglutinin without affecting basolateral transport of vesicular stomatitis virus G protein. Thus, we suggest that caveolin-1 homooligomers play a role in apical transport.
format Text
id pubmed-2141752
institution National Center for Biotechnology Information
language English
publishDate 1998
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21417522008-05-01 Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells Scheiffele, P. Verkade, P. Fra, A.M. Virta, H. Simons, K. Ikonen, E. J Cell Biol Article Abstract. We have studied the biosynthesis and transport of the endogenous caveolins in MDCK cells. We show that in addition to homooligomers of caveolin-1, heterooligomeric complexes of caveolin-1 and -2 are formed in the ER. The oligomers become larger, increasingly detergent insoluble, and phosphorylated on caveolin-2 during transport to the cell surface. In the TGN caveolin-1/-2 heterooligomers are sorted into basolateral vesicles, whereas larger caveolin-1 homooligomers are targeted to the apical side. Caveolin-1 is present on both the apical and basolateral plasma membrane, whereas caveolin-2 is enriched on the basolateral surface where caveolae are present. This suggests that caveolin-1 and -2 heterooligomers are involved in caveolar biogenesis in the basolateral plasma membrane. Anti–caveolin-1 antibodies inhibit the apical delivery of influenza virus hemagglutinin without affecting basolateral transport of vesicular stomatitis virus G protein. Thus, we suggest that caveolin-1 homooligomers play a role in apical transport. The Rockefeller University Press 1998-02-23 /pmc/articles/PMC2141752/ /pubmed/9472032 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Scheiffele, P.
Verkade, P.
Fra, A.M.
Virta, H.
Simons, K.
Ikonen, E.
Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title_full Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title_fullStr Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title_full_unstemmed Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title_short Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells
title_sort caveolin-1 and -2 in the exocytic pathway of mdck cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141752/
https://www.ncbi.nlm.nih.gov/pubmed/9472032
work_keys_str_mv AT scheiffelep caveolin1and2intheexocyticpathwayofmdckcells
AT verkadep caveolin1and2intheexocyticpathwayofmdckcells
AT fraam caveolin1and2intheexocyticpathwayofmdckcells
AT virtah caveolin1and2intheexocyticpathwayofmdckcells
AT simonsk caveolin1and2intheexocyticpathwayofmdckcells
AT ikonene caveolin1and2intheexocyticpathwayofmdckcells