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STUDIES ON ENZYMATIC HISTOCHEMISTRY : XXV. A MICRO METHOD FOR THE DETERMINATION OF CHOLINE ESTERASE AND THE ACTIVITY-PH RELATIONSHIP OF THIS ENZYME

The activity-pH relationship for choline esterase from horse serum, gastric mucosa of the pig, and cat brain was investigated, and an optimum was observed at pH 8.5 in each case. A micro method for the determination of choline esterase was developed capable of measuring the hydrolysis down to the or...

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Detalles Bibliográficos
Autor principal: Glick, David
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1938
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141947/
https://www.ncbi.nlm.nih.gov/pubmed/19873051
Descripción
Sumario:The activity-pH relationship for choline esterase from horse serum, gastric mucosa of the pig, and cat brain was investigated, and an optimum was observed at pH 8.5 in each case. A micro method for the determination of choline esterase was developed capable of measuring the hydrolysis down to the order of that given by 1 x 10(–8) mol of ester.