PURIFICATION OF THROMBIN

1. Under certain conditions crude prothrombin changes to thrombin without the addition of extraneous activators and in the absence of ionic calcium. 2. Thrombin is soluble in 0.45 saturated ammonium sulfate, whereas crude prothrombin is not. 3. Partially purified thrombin is comparatively stable in...

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Detalles Bibliográficos
Autor principal: Milstone, H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1942
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142535/
https://www.ncbi.nlm.nih.gov/pubmed/19873305
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author Milstone, H.
author_facet Milstone, H.
author_sort Milstone, H.
collection PubMed
description 1. Under certain conditions crude prothrombin changes to thrombin without the addition of extraneous activators and in the absence of ionic calcium. 2. Thrombin is soluble in 0.45 saturated ammonium sulfate, whereas crude prothrombin is not. 3. Partially purified thrombin is comparatively stable in concentrated ammonium sulfate solutions at pH 5.2. 4. A method based on the above facts yields a thrombin preparation the specific activity of which is 100 to 175 times the potential specific activity of whole plasma.
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spelling pubmed-21425352008-04-23 PURIFICATION OF THROMBIN Milstone, H. J Gen Physiol Article 1. Under certain conditions crude prothrombin changes to thrombin without the addition of extraneous activators and in the absence of ionic calcium. 2. Thrombin is soluble in 0.45 saturated ammonium sulfate, whereas crude prothrombin is not. 3. Partially purified thrombin is comparatively stable in concentrated ammonium sulfate solutions at pH 5.2. 4. A method based on the above facts yields a thrombin preparation the specific activity of which is 100 to 175 times the potential specific activity of whole plasma. The Rockefeller University Press 1942-05-20 /pmc/articles/PMC2142535/ /pubmed/19873305 Text en Copyright © Copyright, 1942, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Milstone, H.
PURIFICATION OF THROMBIN
title PURIFICATION OF THROMBIN
title_full PURIFICATION OF THROMBIN
title_fullStr PURIFICATION OF THROMBIN
title_full_unstemmed PURIFICATION OF THROMBIN
title_short PURIFICATION OF THROMBIN
title_sort purification of thrombin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142535/
https://www.ncbi.nlm.nih.gov/pubmed/19873305
work_keys_str_mv AT milstoneh purificationofthrombin

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