THE NUCLEOPROTAMINE OF TROUT SPERM

The nucleoprotamine of trout sperm can be extracted completely with 1 M sodium chloride. On reducing the salt concentration to 0.14 M, physiological saline, the nucleoprotamine precipitates in long, fibrous strands. When the nucleoprotamine, dissolved in M NaCl, is dialyzed all the protamine diffuse...

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Detalles Bibliográficos
Autores principales: Pollister, A. W., Mirsky, A. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1946
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142822/
https://www.ncbi.nlm.nih.gov/pubmed/19873480
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author Pollister, A. W.
Mirsky, A. E.
author_facet Pollister, A. W.
Mirsky, A. E.
author_sort Pollister, A. W.
collection PubMed
description The nucleoprotamine of trout sperm can be extracted completely with 1 M sodium chloride. On reducing the salt concentration to 0.14 M, physiological saline, the nucleoprotamine precipitates in long, fibrous strands. When the nucleoprotamine, dissolved in M NaCl, is dialyzed all the protamine diffuses through the membrane leaving behind highly polymerized, protein-free desoxyribose nucleic acid. The nucleoprotamine constitutes 91 per cent of the lipid-free mass of the sperm nucleus. While nucleoprotamine is being extracted by M NaCl a stage is reached at which the sperm chromosomes are clearly visible.
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spelling pubmed-21428222008-04-23 THE NUCLEOPROTAMINE OF TROUT SPERM Pollister, A. W. Mirsky, A. E. J Gen Physiol Article The nucleoprotamine of trout sperm can be extracted completely with 1 M sodium chloride. On reducing the salt concentration to 0.14 M, physiological saline, the nucleoprotamine precipitates in long, fibrous strands. When the nucleoprotamine, dissolved in M NaCl, is dialyzed all the protamine diffuses through the membrane leaving behind highly polymerized, protein-free desoxyribose nucleic acid. The nucleoprotamine constitutes 91 per cent of the lipid-free mass of the sperm nucleus. While nucleoprotamine is being extracted by M NaCl a stage is reached at which the sperm chromosomes are clearly visible. The Rockefeller University Press 1946-11-20 /pmc/articles/PMC2142822/ /pubmed/19873480 Text en Copyright © Copyright, 1946, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Pollister, A. W.
Mirsky, A. E.
THE NUCLEOPROTAMINE OF TROUT SPERM
title THE NUCLEOPROTAMINE OF TROUT SPERM
title_full THE NUCLEOPROTAMINE OF TROUT SPERM
title_fullStr THE NUCLEOPROTAMINE OF TROUT SPERM
title_full_unstemmed THE NUCLEOPROTAMINE OF TROUT SPERM
title_short THE NUCLEOPROTAMINE OF TROUT SPERM
title_sort nucleoprotamine of trout sperm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142822/
https://www.ncbi.nlm.nih.gov/pubmed/19873480
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