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CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of...
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1948
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147134/ https://www.ncbi.nlm.nih.gov/pubmed/18891150 |
| Sumario: | Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of fine needles usually produced in ammonium sulfate solution. Chymotrypsin yields fine needles in the presence of alcohol and rhombohedrons in the presence of ammonium sulfate. The enzymatic properties of the crystals formed in the presence of alcohol are identical with those of the crystals isolated in the presence of ammonium sulfate. |
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