CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL

Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of...

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Detalles Bibliográficos
Autor principal: Kunitz, M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1948
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147134/
https://www.ncbi.nlm.nih.gov/pubmed/18891150
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author Kunitz, M.
author_facet Kunitz, M.
author_sort Kunitz, M.
collection PubMed
description Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of fine needles usually produced in ammonium sulfate solution. Chymotrypsin yields fine needles in the presence of alcohol and rhombohedrons in the presence of ammonium sulfate. The enzymatic properties of the crystals formed in the presence of alcohol are identical with those of the crystals isolated in the presence of ammonium sulfate.
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spelling pubmed-21471342008-04-23 CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL Kunitz, M. J Gen Physiol Article Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of fine needles usually produced in ammonium sulfate solution. Chymotrypsin yields fine needles in the presence of alcohol and rhombohedrons in the presence of ammonium sulfate. The enzymatic properties of the crystals formed in the presence of alcohol are identical with those of the crystals isolated in the presence of ammonium sulfate. The Rockefeller University Press 1948-11-20 /pmc/articles/PMC2147134/ /pubmed/18891150 Text en Copyright © Copyright, 1948, The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kunitz, M.
CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title_full CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title_fullStr CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title_full_unstemmed CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title_short CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL
title_sort crystallization of salt-free chymotrypsinogen and chymotrypsin from solution in dilute ethyl alcohol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147134/
https://www.ncbi.nlm.nih.gov/pubmed/18891150
work_keys_str_mv AT kunitzm crystallizationofsaltfreechymotrypsinogenandchymotrypsinfromsolutionindiluteethylalcohol

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