PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES : V. FURTHER OBSERVATIONS ON THE RELATIONSHIP BETWEEN VIRUS RELEASE AND CELLULAR LYSIS

1. In the synthetic medium of Fildes contaming hydrolyzed casein virus release is not correlated with observable cellular lysis under conditions of a very low multiple infection. 2. In cells with a high multiple infection, lysis occurs much sooner than in cells with a low multiple infection and virus release is correlated with cellular lysis. The experiments indicate that it is the virus particle itself which accelerates lysis under these conditions. 3. A non-dialyzable fraction has been isolated from yeast, the addition of which results in cellular lysis occurring at a sooner than usual interval and being correlated with virus release in cells having a very low multiple infection. 4. By varying the concentration of the yeast fraction, it is possible to lyse the cells at varying times under conditions of a low multiple infection.