STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER

Three enzymic phenomena, inactivation by oxygen, Q (O(O2)) measurements of a dehydrogenase system, and hydrolysis of acetyl phosphate by a phosphatase of Clostridium kluyveri, were studied by means of the differential microrespirometer. Respiration of yeast cells was also measured with the same inst...

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Detalles Bibliográficos
Autores principales: Entner, Nathan, Kirk, Paul L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1951
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147230/
https://www.ncbi.nlm.nih.gov/pubmed/14824509
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author Entner, Nathan
Kirk, Paul L.
author_facet Entner, Nathan
Kirk, Paul L.
author_sort Entner, Nathan
collection PubMed
description Three enzymic phenomena, inactivation by oxygen, Q (O(O2)) measurements of a dehydrogenase system, and hydrolysis of acetyl phosphate by a phosphatase of Clostridium kluyveri, were studied by means of the differential microrespirometer. Respiration of yeast cells was also measured with the same instrument. All results obtained in the four types of study agreed closely with earlier results obtained with the Warburg apparatus. The amount of sample needed was of the order of 1/100 to 1/200 of that necessary for comparable study with the Warburg apparatus. The advantages of the instrument in enzyme isolation and purification studies are discussed.
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spelling pubmed-21472302008-04-23 STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER Entner, Nathan Kirk, Paul L. J Gen Physiol Article Three enzymic phenomena, inactivation by oxygen, Q (O(O2)) measurements of a dehydrogenase system, and hydrolysis of acetyl phosphate by a phosphatase of Clostridium kluyveri, were studied by means of the differential microrespirometer. Respiration of yeast cells was also measured with the same instrument. All results obtained in the four types of study agreed closely with earlier results obtained with the Warburg apparatus. The amount of sample needed was of the order of 1/100 to 1/200 of that necessary for comparable study with the Warburg apparatus. The advantages of the instrument in enzyme isolation and purification studies are discussed. The Rockefeller University Press 1951-03-20 /pmc/articles/PMC2147230/ /pubmed/14824509 Text en Copyright © Copyright, 1951, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Entner, Nathan
Kirk, Paul L.
STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title_full STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title_fullStr STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title_full_unstemmed STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title_short STUDY OF SOME ENZYME SYSTEMS BY MEANS OF THE DIFFERENTIAL MICRORESPIROMETER
title_sort study of some enzyme systems by means of the differential microrespirometer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147230/
https://www.ncbi.nlm.nih.gov/pubmed/14824509
work_keys_str_mv AT entnernathan studyofsomeenzymesystemsbymeansofthedifferentialmicrorespirometer
AT kirkpaull studyofsomeenzymesystemsbymeansofthedifferentialmicrorespirometer

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