METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT

1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect...

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Detalles Bibliográficos
Autores principales: Kurnick, N. B., Foster, Marilee
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1950
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147252/
https://www.ncbi.nlm.nih.gov/pubmed/14824487
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author Kurnick, N. B.
Foster, Marilee
author_facet Kurnick, N. B.
Foster, Marilee
author_sort Kurnick, N. B.
collection PubMed
description 1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mµ in the pH range 3.5–7.8, whereas the free dye fades markedly above pH 5.0.
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spelling pubmed-21472522008-04-23 METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT Kurnick, N. B. Foster, Marilee J Gen Physiol Article 1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mµ in the pH range 3.5–7.8, whereas the free dye fades markedly above pH 5.0. The Rockefeller University Press 1950-11-20 /pmc/articles/PMC2147252/ /pubmed/14824487 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kurnick, N. B.
Foster, Marilee
METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title_full METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title_fullStr METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title_full_unstemmed METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title_short METHYL GREEN : III. REACTION WITH DESOXYRIBONUCLEIC ACID, STOICHIOMETRY, AND BEHAVIOR OF THE REACTION PRODUCT
title_sort methyl green : iii. reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147252/
https://www.ncbi.nlm.nih.gov/pubmed/14824487
work_keys_str_mv AT kurnicknb methylgreeniiireactionwithdesoxyribonucleicacidstoichiometryandbehaviorofthereactionproduct
AT fostermarilee methylgreeniiireactionwithdesoxyribonucleicacidstoichiometryandbehaviorofthereactionproduct

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