THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA

1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunologica...

Descripción completa

Detalles Bibliográficos
Autores principales: Allfrey, V., Stern, H., Mirsky, A. E., Saetren, H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1952
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147330/
https://www.ncbi.nlm.nih.gov/pubmed/14898034
_version_ 1782144414000349184
author Allfrey, V.
Stern, H.
Mirsky, A. E.
Saetren, H.
author_facet Allfrey, V.
Stern, H.
Mirsky, A. E.
Saetren, H.
author_sort Allfrey, V.
collection PubMed
description 1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.
format Text
id pubmed-2147330
institution National Center for Biotechnology Information
language English
publishDate 1952
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21473302008-04-23 THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA Allfrey, V. Stern, H. Mirsky, A. E. Saetren, H. J Gen Physiol Article 1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed. The Rockefeller University Press 1952-01-20 /pmc/articles/PMC2147330/ /pubmed/14898034 Text en Copyright © Copyright, 1952, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Allfrey, V.
Stern, H.
Mirsky, A. E.
Saetren, H.
THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title_full THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title_fullStr THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title_full_unstemmed THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title_short THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA
title_sort isolation of cell nuclei in non-aqueous media
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147330/
https://www.ncbi.nlm.nih.gov/pubmed/14898034
work_keys_str_mv AT allfreyv theisolationofcellnucleiinnonaqueousmedia
AT sternh theisolationofcellnucleiinnonaqueousmedia
AT mirskyae theisolationofcellnucleiinnonaqueousmedia
AT saetrenh theisolationofcellnucleiinnonaqueousmedia
AT allfreyv isolationofcellnucleiinnonaqueousmedia
AT sternh isolationofcellnucleiinnonaqueousmedia
AT mirskyae isolationofcellnucleiinnonaqueousmedia
AT saetrenh isolationofcellnucleiinnonaqueousmedia

Ejemplares similares