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PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
1. A method for the preparation of 8 to 10 liter quantities of T(2) virus lysates, titering 2 to 5 x 10(11) infectious units per ml. has been described. 2. Procedures have been developed for the concentration and purification of virus to a high specific infectivity. No fractionation procedure of the...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1952
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147345/ https://www.ncbi.nlm.nih.gov/pubmed/12981232 |
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author | Herriott, Roger M. Barlow, James L. |
author_facet | Herriott, Roger M. Barlow, James L. |
author_sort | Herriott, Roger M. |
collection | PubMed |
description | 1. A method for the preparation of 8 to 10 liter quantities of T(2) virus lysates, titering 2 to 5 x 10(11) infectious units per ml. has been described. 2. Procedures have been developed for the concentration and purification of virus to a high specific infectivity. No fractionation procedure of the several used succeeded in further raising the specific infectivity of these purified preparations. 3. Some of the general properties of the better preparations have been determined. They exhibited titers of 2 x 10(15) infective units per gm. of material or 1.2 x 10(16) per gm. of nitrogen. 4. A study of the distribution of nitrogen among the various fractions of the virus showed that about 6 per cent of the total nitrogen is soluble in 4 per cent trichloracetic acid; that the protein nitrogen is about 40 per cent of the total and the nucleic acid nitrogen is 53 per cent. At least 96 per cent of the total phosphorus is in the nucleic acid fraction. Less than 0.5 per cent quantities of lipid and PNA were found. |
format | Text |
id | pubmed-2147345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1952 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21473452008-04-23 PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2) Herriott, Roger M. Barlow, James L. J Gen Physiol Article 1. A method for the preparation of 8 to 10 liter quantities of T(2) virus lysates, titering 2 to 5 x 10(11) infectious units per ml. has been described. 2. Procedures have been developed for the concentration and purification of virus to a high specific infectivity. No fractionation procedure of the several used succeeded in further raising the specific infectivity of these purified preparations. 3. Some of the general properties of the better preparations have been determined. They exhibited titers of 2 x 10(15) infective units per gm. of material or 1.2 x 10(16) per gm. of nitrogen. 4. A study of the distribution of nitrogen among the various fractions of the virus showed that about 6 per cent of the total nitrogen is soluble in 4 per cent trichloracetic acid; that the protein nitrogen is about 40 per cent of the total and the nucleic acid nitrogen is 53 per cent. At least 96 per cent of the total phosphorus is in the nucleic acid fraction. Less than 0.5 per cent quantities of lipid and PNA were found. The Rockefeller University Press 1952-09-20 /pmc/articles/PMC2147345/ /pubmed/12981232 Text en Copyright © Copyright, 1952, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Herriott, Roger M. Barlow, James L. PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2) |
title | PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
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title_full | PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
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title_fullStr | PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
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title_full_unstemmed | PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
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title_short | PREPARATION, PURIFICATION, AND PROPERTIES OF E. COLI VIRUS T(2)
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title_sort | preparation, purification, and properties of e. coli virus t(2) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147345/ https://www.ncbi.nlm.nih.gov/pubmed/12981232 |
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